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APOBEC3B antibody, N-term

Anti-APOBEC3B antibody, N-term used in Western Blot (WB). GTX46485
Anti-APOBEC3B antibody, N-term used in Western Blot (WB). GTX46485

Cat. No. GTX46485

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC

Reactivity

 Human
Package
50 μg ($419)
See all APOBEC3B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
IHC Assay dependent
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

HepG2

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a N-terminal region of Human APOBEC3B

Purification

Affinity Purified

Conjugation

Unconjugated

RRID

AB_11163410

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

apolipoprotein B mRNA editing enzyme catalytic subunit 3B , A3B , APOBEC1L , ARCD3 , ARP4 , DJ742C19.2 , PHRBNL , bK150C2.2

Cellular Localization

Nucleus

Background

This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. A hybrid gene results from the deletion of approximately 29.5 kb of sequence between this gene, APOBEC3B, and the adjacent gene APOBEC3A. The breakpoints of the deletion are within the two genes, so the deletion allele is predicted to have the promoter and coding region of APOBEC3A, but the 3' UTR of APOBEC3B. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]

Database

Research Area

DATA IMAGES

Anti-APOBEC3B antibody, N-term used in Western Blot (WB). GTX46485

GTX46485 WB Image

WB analysis of HepG2 cells using GTX46485 APOBEC3B antibody at 0.2-1μg/ml.

Anti-APOBEC3B antibody, N-term used in Western Blot (WB). GTX46485

GTX46485 WB Image

WB analysis of HepG2 cells using GTX46485 APOBEC3B antibody at 1.0μg/ml.
Lane A : primary antibody
Lane B : primary antibody + Blocking peptide

REFERENCE

Application Reference

Gwak M et al. Tumori 2014; 100 (4) : 112e-7e Expression of DNA cytosine deaminase APOBEC3 proteins, a potential source for producing mutations, in gastric, colorectal and prostate cancers.
Application : IHC
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419