APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 - 2 μg/mL |
| 5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Rat Small Intestine Tissue Lysate(GTX24038) , Human Small Intestine Tissue Slide
Product Note
At least three isoforms of APOBEC3B are known to exist; this antibody will detect all three. APOBEC3B antibody may cross-react with APOBEC3D.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
APOBEC3B antibody was raised against a 14 amino acid peptide near the amino terminus of human APOBEC3B.The immunogen is located within amino acids 20 - 70 of APOBEC3B.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
RRID
AB_2887555
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
apolipoprotein B mRNA editing enzyme catalytic subunit 3B , A3B , APOBEC1L , ARCD3 , ARP4 , DJ742C19.2 , PHRBNL , bK150C2.2
Cellular Localization
Nucleus
Background
This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. A hybrid gene results from the deletion of approximately 29.5 kb of sequence between this gene, APOBEC3B, and the adjacent gene APOBEC3A. The breakpoints of the deletion are within the two genes, so the deletion allele is predicted to have the promoter and coding region of APOBEC3A, but the 3' UTR of APOBEC3B. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]
Database
Research Area
DATA IMAGES
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GTX17214 IHC-P Image
IHC-P analysis of human small intestine tissue using GTX17214 APOBEC3B antibody.
Working concentration : 20 μg/ml
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GTX17214 WB Image
WB analysis of rat small intestine tissue lysate using GTX17214 APOBEC3B antibody.
Working concentration : 1 μg/ml
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GTX17214 IHC-P Image
IHC-P analysis of human small intestine tissue using GTX17214 APOBEC3B antibody.
Working concentration : 5 μg/ml
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GTX17214 WB Image
The data was published in the journal Biochem Biophys Rep in 2018. PMID: 30417129
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REFERENCE
REVIEW
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