GeneTex
United States (US)

APOBEC3C antibody

KO/KD
GTX102164_40968_WB_19011506_558.jpg

Cat No. GTX102164

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP Assay dependent
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

NCI-H929

Specificity/Sensitivity

Knockdown/Knockout validation was supported by references (PMID:26990993)

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human APOBEC3C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Apolipoprotein B Mrna Editing Enzyme Catalytic Subunit 3C,A3C,Apobec1L,Ardc2,Ardc4,Arp5,Pbi,Bk150C2.3,Apobec3C

Background

This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102164_40968_WB_19011506_558.jpg

GTX102164 WB Image

Sample (30 ug of whole cell lysate)
A: NCI-H929
12% SDS PAGE
GTX102164 diluted at 1:2000

REFERENCE
REVIEW

APOBEC3C antibody Cat No. GTX102164

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 11-Oct-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : AGS cells
Amount used : 20 μg
Blocking : 5% BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Lane Description : Lane 1: Wild-type (WT), Lane 2: APOBEC3C knockout (KO)
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169