GeneTex
United States (US)

APOBEC3G antibody, C-term

GTX88548_2579_WB_18121411_714.jpg
GTX88548_769_IHC-P_18121411_376.jpg
GTX88548_770_IHC-P_18121411_703.jpg

Cat No. GTX88548

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1.5μg/ml
IHC-P 2.5μg/ml
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88548-PEP).
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50mg/ml

Antigen Species

Human

Immunogen

Peptide with sequence C-DEHSQDLSGRLR, from the C Terminus of the protein sequence according to NP_068594.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MDS019 Antibody , DJ494G10.1 Antibody , A3G Antibody , ARCD Antibody , ARP9 Antibody , CEM15 Antibody , BK150C2.7 Antibody

Cellular Localization

Mainly cytoplasmic. Small amount are found in the nucleus. During HIV-1 infection, virion-encapsidated in absence of HIV-1 VIF.

Background

This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. The protein encoded by this gene has been found to be a specific inhibitor of human immunodeficiency virus-1 (HIV-1) infectivity. [provided by RefSeq, Jul 2008]
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Database

Research Area

DATA IMAGES
GTX88548_2579_WB_18121411_714.jpg

GTX88548 WB Image

WB analysis of Daudi lysate using GTX88548 APOBEC3G antibody, C-term.
Dilution : 0.5µg/ml
Loading : 35µg protein in RIPA buffer

GTX88548_769_IHC-P_18121411_376.jpg

GTX88548 IHC-P Image

IHC-P analysis of human kidney using GTX88548 APOBEC3G antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5µg/ml

GTX88548_770_IHC-P_18121411_703.jpg

GTX88548 IHC-P Image

IHC-P analysis of human testis using GTX88548 APOBEC3G antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5µg/ml