APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 5 μg/mL |
| 1 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Caco-2 Cell Lysate(GTX23950) , Human Spleen Tissue Lysate(GTX27921) , Human Spleen Tissue Slide
Product Note
APOBEC3G antibody will also detect the APOBEC3F isoform.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
APOBEC3G antibody was raised against a synthetic peptide corresponding to 15 amino acids near the amino-terminus of human APOBEC3G.The immunogen is located within the first 50 amino acids of APOBEC3G.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
apolipoprotein B mRNA editing enzyme catalytic subunit 3G , A3G , ARCD , ARP-9 , ARP9 , CEM-15 , CEM15 , MDS019 , bK150C2.7 , dJ494G10.1
Cellular Localization
Cytoplasm,Nucleus
Background
The Apoliprotein B mRNA-editing, enzyme-catalytic, polypeptide-like (APOBEC) 3 is a multi-isoform member of the cytidine deaminase family of enzymes that act on monomeric nucleoside and nucleotide substrates (1). Similar to TRIM5alpha which targets incoming retroviral capsids, APOBEC3 plays a major role in cellular defense against retroviral infection (2,3) as at least two isoforms, APOBEC3G and to a lesser extent APOBEC3F, can be incorporated HIV-1 virions and induce hypermutation in the newly synthesized viral DNA and thus destabilize the viral genome (4,5). This innate mechanism of retroviral resistance is counteracted by the HIV-1 Vif protein by inducing the ubiquitization and degradation of APOBEC3G; a single amino acid substitution (D128K) blocks APOBEC3G depletion without affecting its inhibitory activity (6).
Database
Research Area
DATA IMAGES
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GTX85084 WB Image
WB analysis of Caco-2 cell lysate in the (A)absence and (B) presence of blocking peptide using GTX85084 APOBEC3G antibody.
Working concentration : 5 μg/ml
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GTX85084 IHC-P Image
IHC-P analysis of human spleen tissue using GTX85084 APOBEC3G antibody.
Working concentration : 1 μg/ml
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REFERENCE
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REVIEW
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