APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:1000-1:10000 |
Not tested in other applications.
Calculated MW
Positive Control
U87-MG , SK-N-SH , IMR32 , SK-N-AS , human cerebrospinal fluid , mouse brain , rat brian
Predict Reactivity
Rabbit, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.46 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the Extracellular domain of human APP. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10620428
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
amyloid beta precursor protein , AAA , ABETA , ABPP , AD1 , APPI , CTFgamma , CVAP , PN-II , PN2 , preA4
Cellular Localization
Membrane , Membrane , clathrin-coated pit
Background
This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX112677 ICC/IF Image
APP antibody detects APP protein at cytoplasm by immunofluorescent analysis.
Sample: SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: APP protein stained by APP antibody (GTX112677) diluted at 1:100.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at 1:200.
Blue: Hoechst 33342 staining.
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GTX112677 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody (GTX112677) diluted at 1:500.
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GTX112677 WB Image
Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody (GTX112677) diluted at 1:500.
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GTX112677 IHC-P Image
APP antibody detects APP protein at cytoplasm in Rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded Rat brain.
APP antibody (GTX112677) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX112677 WB Image
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody (GTX112677) diluted at 1:500.
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GTX112677 WB Image
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody (GTX112677) diluted at 1:500.
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GTX112677 ICC/IF Image
APP antibody detects APP protein at cytoplasm by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: APP protein stained by APP antibody (GTX112677) diluted at 1:100.
Blue: Hoechst 33342 staining.
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GTX112677 ELISA Image
Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX112677 as capture antibody at concentration of 5 μg/mL and a mouse monoclonal anti-APP antibody as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.
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REFERENCE
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