GeneTex
United States (US)

APP antibody

GTX112677_40177_20180907_ELISA_B_18121410_290.jpg
GTX112677_40177_20160204_WB_R_brain_18121410_435.jpg
GTX112677_40177_20160520_IFA_2_18121410_731.jpg
GTX112677_40177_20160204_WB_18121410_313.jpg
GTX112677_40177_20160204_WB_H_cerebrospinal fluid_18121410_591.jpg
GTX112677_40177_20160520_IFA_18121410_629.jpg
GTX112677_40177_20160106_IHC-P_R_18121410_841.jpg
GTX112677_40177_20160204_WB_M_brain_18121410_329.jpg

Cat No. GTX112677

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
ELISA 1:1000-1:10000
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS , human cerebrospinal fluid , mouse brain , rat brian

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

0.46mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human APP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AAA antibody, ABETA antibody, ABPP antibody, AD1 antibody, APPI antibody, CTFgamma antibody, CVAP antibody, PN2 antibody, APP antibody, alzheimer disease amyloid protein antibody, beta-amyloid peptide antibody, amyloid beta A4 protein antibody, peptidase nexin-II antibody, protease nexin-II antibody, cerebral vascular amyloid peptide antibody, preA4 antibody, amyloid beta (A4) precursor protein antibody, beta Amyloid antibody

Cellular Localization

Membrane , Membrane , clathrin-coated pit

Background

This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX112677_40177_20180907_ELISA_B_18121410_290.jpg

GTX112677 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX112677 as capture antibody at concentration of 5 µg/mL and a mouse monoclonal anti-APP antibody as detection antibody at concentration of 1 µg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX112677_40177_20160204_WB_R_brain_18121410_435.jpg

GTX112677 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody (GTX112677) diluted at 1:500.

GTX112677_40177_20160520_IFA_2_18121410_731.jpg

GTX112677 ICC/IF Image

APP antibody detects APP protein at cytoplasm by immunofluorescent analysis.
Sample: SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: APP protein stained by APP antibody (GTX112677) diluted at 1:100.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at 1:200.
Blue: Hoechst 33342 staining.

GTX112677_40177_20160204_WB_18121410_313.jpg

GTX112677 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody (GTX112677) diluted at 1:500.

GTX112677_40177_20160204_WB_H_cerebrospinal fluid_18121410_591.jpg

GTX112677 WB Image

Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody (GTX112677) diluted at 1:500.

GTX112677_40177_20160520_IFA_18121410_629.jpg

GTX112677 ICC/IF Image

APP antibody detects APP protein at cytoplasm by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: APP protein stained by APP antibody (GTX112677) diluted at 1:100.
Blue: Hoechst 33342 staining.

GTX112677_40177_20160106_IHC-P_R_18121410_841.jpg

GTX112677 IHC-P Image

APP antibody detects APP protein at cytoplasm in Rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded Rat brain.
APP antibody (GTX112677) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX112677_40177_20160204_WB_M_brain_18121410_329.jpg

GTX112677 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody (GTX112677) diluted at 1:500.

SDS
Glycerol.pdf
Thimerosal.pdf