GeneTex
United States (US)

APP (phospho Thr743) antibody

GTX32308_20190131_WB_19013102_764.jpg
GTX32308_20190131_IHC-P_19013102_686.jpg

Cat No. GTX32308

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

87 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of APP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Amyloid Beta Precursor Protein,Aaa,Abeta,Abpp,Ad1,Appi,Ctfgamma,Cvap,Pn-Ii,Pn2,Prea4,App

Cellular Localization

Clathrin-coated pit,Membrane

Background

This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Aug 2014]

Database

Research Area

DATA IMAGES
GTX32308_20190131_WB_19013102_764.jpg

GTX32308 WB Image

WB analysis of colchicine-treated MCF7 (A), mouse liver (B), PC12 (C) whole cell lysates using GTX32308 APP (phospho Thr743) antibody.

GTX32308_20190131_IHC-P_19013102_686.jpg

GTX32308 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32308 APP (phospho Thr743) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Package List Price ($)
$ 319