GeneTex
United States (US)

APPL1 antibody [N1N2], N-term

GTX115786_40296_WB_18121410_992.jpg
GTX115786_40296_IHC_18121410_893.jpg
GTX115786_40296_IHC_M_18121410_853.jpg

Cat No. GTX115786

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

U87-MG , MCF-7

Predict Reactivity

Zebrafish, Bovine, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human APPL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

APPL antibody, DIP13alpha antibody, APPL1 antibody, DCC-interacting protein 13-alpha antibody, "adaptor protein containing pH domain, PTB domain and leucine zipper motif 1 antibody", "adapter protein containing PH domain, PTB domain and leucine zipper motif 1 antibody", AKT2 interactor antibody, signaling adaptor protein DIP13alpha antibody, dip13-alpha antibody, "adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 antibody"

Background

The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX115786_40296_WB_18121410_992.jpg

GTX115786 WB Image

Sample (30 ug of whole cell lysate)
A: MCF-7
7.5% SDS PAGE
GTX115786 diluted at 1:1000

GTX115786_40296_IHC_18121410_893.jpg

GTX115786 IHC-P Image

Immunohistochemical analysis of paraffin-embedded AZ521 xenograft, using APPL1(GTX115786) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115786_40296_IHC_M_18121410_853.jpg

GTX115786 IHC-P Image

APPL1 antibody [N1N2], N-term detects APPL1 protein at cytosol on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
APPL1 antibody [N1N2], N-term (GTX115786) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf