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APPL1 antibody [N1N2], N-term

Anti-APPL1 antibody [N1N2], N-term used in Western Blot (WB). GTX115786
Anti-APPL1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX115786
Anti-APPL1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX115786

Cat. No. GTX115786

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all APPL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

MCF-7

Predict Reactivity

Zebrafish, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human APPL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619598

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 , APPL , DIP13alpha , MODY14

Background

The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-APPL1 antibody [N1N2], N-term used in Western Blot (WB). GTX115786

GTX115786 WB Image

Sample (30 ug of whole cell lysate)
A: MCF-7
7.5% SDS PAGE
GTX115786 diluted at 1:1000

Anti-APPL1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX115786

GTX115786 IHC-P Image

Immunohistochemical analysis of paraffin-embedded AZ521 xenograft, using APPL1(GTX115786) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-APPL1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX115786

GTX115786 IHC-P Image

APPL1 antibody [N1N2], N-term detects APPL1 protein at cytosol on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
APPL1 antibody [N1N2], N-term (GTX115786) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169