APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
5 - 10 μg/mL |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
K562 Cell Lysate(GTX27911)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
APRIL antibody was raised against a synthetic peptide corresponding to amino acids near the center of human APRIL.The immunogen is located within amino acids 50 - 100 of APRIL.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
TNF superfamily member 13 , APRIL , CD256 , TALL-2 , TALL2 , TNLG7B , TRDL-1 , UNQ383/PRO715 , ZTNF2
Cellular Localization
Secreted
Background
The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF17/BCMA, a member of the TNF receptor family. This protein and its receptor are both found to be important for B cell development. In vitro experiments suggested that this protein may be able to induce apoptosis through its interaction with other TNF receptor family proteins such as TNFRSF6/FAS and TNFRSF14/HVEM. Alternative splicing results in multiple transcript variants. Some transcripts that skip the last exon of the upstream gene (TNFSF12) and continue into the second exon of this gene have been identified; such read-through transcripts are contained in GeneID 407977, TNFSF12-TNFSF13. [provided by RefSeq, Oct 2010]
Database
Research Area
DATA IMAGES
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GTX31650 WB Image
WB analysis of K562 cell lysate using GTX31650 APRIL antibody. Working concentration : (A) 5 and (B) 10 μg/ml
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REFERENCE
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REVIEW
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