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ARA9 antibody [N1C3]

Anti-ARA9 antibody [N1C3] used in Western Blot (WB). GTX110665
Anti-ARA9 antibody [N1C3] used in Western Blot (WB). GTX110665
Anti-ARA9 antibody [N1C3] used in Western Blot (WB). GTX110665
Anti-ARA9 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110665
Anti-ARA9 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX110665

Cat. No. GTX110665

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ARA9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

HeLa , Molt-4 , Raji , Mouse brain , PC-12 , Rat2

Predict Reactivity

Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ARA9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949611

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aryl hydrocarbon receptor interacting protein , ARA9 , FKBP16 , FKBP37 , PITA1 , SMTPHN , XAP-2 , XAP2

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a receptor for aryl hydrocarbons and a ligand-activated transcription factor. The encoded protein is found in the cytoplasm as part of a multiprotein complex, but upon binding of ligand is transported to the nucleus. This protein can regulate the expression of many xenobiotic metabolizing enzymes. Also, the encoded protein can bind specifically to and inhibit the activity of hepatitis B virus. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ARA9 antibody [N1C3] used in Western Blot (WB). GTX110665

GTX110665 WB Image

ARA9 antibody [N1C3] detects ARA9 protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg Molt-4 whole cell lysate/extract
C. 30 μg Raji whole cell lysate/extract
10 % SDS-PAGE
ARA9 antibody [N1C3] (GTX110665) dilution: 1:5000

Anti-ARA9 antibody [N1C3] used in Western Blot (WB). GTX110665

GTX110665 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX110665 diluted at 1:1000

Anti-ARA9 antibody [N1C3] used in Western Blot (WB). GTX110665

GTX110665 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ARA9 antibody [N1C3] (GTX110665) diluted at 1:500.

Anti-ARA9 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110665

GTX110665 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ARA9 (GTX110665) antibody at 1:200 dilution.

Anti-ARA9 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX110665

GTX110665 IHC-P Image

ARA9 antibody [N1C3] detects ARA9 protein at cytoplasm on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
ARA9 antibody [N1C3] (GTX110665) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Song D et al. J Biol Chem 2013; 288 (14) : 9662-74 Prolyl hydroxylase domain protein 2 (PHD2) binds a Pro-Xaa-Leu-Glu motif, linking it to the heat shock protein 90 pathway.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169