GeneTex
United States (US)

ARD1A antibody

KO/KD
GTX125971_40786_WB_18121410_648.jpg
GTX125971_40786_WB_M_18121410_978.jpg
GTX125971_40786_IHC_18121410_807.jpg
GTX125971_40786_20141231_IP_18121410_927.jpg
GTX125971_40787_IFA_18121410_808.jpg

Cat No. GTX125971

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

H1299 transfected scramble siRNA , H1299 transfected ARDIA siRNA , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12

Predict Reactivity

Rat, Rabbit, Bovine, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human ARD1A Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

N(Alpha)-Acetyltransferase 10, Nata Catalytic Subunit,Ard1,Ard1A,Ard1P,Dxs707,Mcops1,Natd,Ogdns,Te2,Hard1,Naa10

Cellular Localization

Cytoplasm , Nucleus

Background

N-alpha-acetylation is one of the most common protein modifications that occurs during protein synthesis and involves the transfer of an acetyl group from acetyl-coenzyme A to the protein alpha-amino group. ARD1A, together with NATH (NARG1; MIM 608000), is part of a major N-alpha-acetyltransferase complex responsible for alpha-acetylation of proteins and peptides (Sanchez-Puig and Fersht, 2006 [PubMed 16823041]).[supplied by OMIM]

Database

DATA IMAGES
GTX125971_40786_WB_18121410_648.jpg

GTX125971 WB Image

KO/KD

Sample (30 µg of whole cell lysate)
A: H1299 transfected scramble siRNA
B: H1299 transfected ARDIA siRNA
12% SDS PAGE
GTX125971 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX125971_40786_WB_M_18121410_978.jpg

GTX125971 WB Image

ARD1A antibody detects ARD1A protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and blotted with ARD1A antibody (GTX125971) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX125971_40786_IHC_18121410_807.jpg

GTX125971 IHC-P Image

Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using ARD1A(GTX125971) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX125971_40786_20141231_IP_18121410_927.jpg

GTX125971 IP Image

Immunoprecipitation of ARD1A protein from HeLa whole cell extracts using 5 μg of ARD1A antibody (GTX125971).
Western blot analysis was performed using ARD1A antibody (GTX125971).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX125971_40787_IFA_18121410_808.jpg

GTX125971 ICC/IF Image

ARD1A antibody detects NAA10 protein at Cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in -20℃ 100% MeOH for 5 min.
Green: NAA10 protein stained by ARD1A antibody (GTX125971) diluted at 1:500.
Blue: Hoechst 33343 staining.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169