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ARF1/3 antibody

Cat No. GTX12418

Host

Sheep

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Mouse
Package
25 μl ($289)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2,000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

mouse brain cells

PROPERTIES

Form

Liquid

Buffer

0.1 M Glycine buffer, 0.1% EACA, 0.01% benzamidine, 1 mM EDTA, 0.1% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

peptide corresponding to amino acids 98-112. Selected for maximal sequence homology with human ARF 1 and 3, but minimal sequence homology to other known ARFs.

Purification

IgG fraction

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ADP ribosylation factor 1 , PVNH8

Cellular Localization

Golgi apparatus,Membrane

Background

The ADP-ribosylation factor (Arf) family comprises a group of structurally and functionally conserved 21 kDa proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. Arf is involved in intracellular protein traffic to and within the Golgi complex. Arf has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D. The Arf family is divided functionally into the Arf and the Arf-like (Arl) proteins. Arfs share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily.

Database

Research Area

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf