ARF1/ARF3/ARF5/ARF6 antibody [1D9]

Cat. No. GTX22806

GTX22806 ICC/IF Image

ICC/IF analysis of U251 cells using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

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GTX22806 FCM Image

FACS analysis of MCF-7 cells using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9] compared to an isotype control (blue).
Dilution : 1:80 for 60 min at room temperature
Fixation : 2% paraformaldehyde

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GTX22806 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

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GTX22806 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

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GTX22806 FCM Image

FACS analysis of 3T3 cells using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9] compared to an isotype control (blue).
Dilution : 1:80 for 60 min at room temperature
Fixation : 2% paraformaldehyde

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GTX22806 WB Image

WB analysis of canine heart extract using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9].

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GTX22806 IHC-P Image

IHC-P analysis of human liver tissue using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:50

7 / 10

GTX22806 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:50

8 / 10

GTX22806 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

9 / 10

GTX22806 FCM Image

FACS analysis of Hela cells using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9] compared to an isotype control (blue).
Dilution : 1:80 for 60 min at room temperature
Fixation : 2% paraformaldehyde

10 / 10

This ARF1/ARF3/ARF5/ARF6 antibody [1D9] (GTX22806) is a mouse monoclonal antibody validated for detection of ARF1/ARF3/ARF5/ARF6 by WB, ICC/IF, IHC-P, FCM, IP, ELISA, and IHC. Reactive to human, mouse, rat, and other species.

Host

Mouse
Clonality

Monoclonal
Clone Name

1D9
Isotype

IgG1
Applications

WB ICC/IF IHC-P FCM IP ELISA IHC
Reactivity

Human, Mouse, Rat, Bovine, Dog, Hamster, Primate