APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000 |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone 1B3 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ARF3 protein.
Purification
Protein G Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ADP ribosylation factor 1 , ARF1
Cellular Localization
Golgi apparatus,Cytoplasm,Perinuclear region,Cell junction,Synapse,Synaptosome,Postsynaptic cell membrane,Postsynaptic density,Membrane
Background
ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX57579 WB Image
WB analysis of various samples using GTX57579 ARF1 antibody. Lane 1 : HeLa whole cell lysate Lane 2 : HepG2 whole cell lysate Lane 3 : Brain tissue lysate Lane 4 : MCF whole cell lysate Loading : 40 μg Dilution : 1:1000
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GTX57579 FACS Image
FACS analysis of MCF7 cells using GTX57579 ARF1 antibody. Cell Number: 1 x 10⁶ cells Primary antibody: Red line Antibody amount: 2-5 μg
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GTX57579 ICC/IF Image
ICC/IF analysis of U87-MG cells using GTX57579 ARF1 antibody. Blue: DAPI Green: Primary antibody Dilution: 1:100
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GTX57579 ICC/IF Image
ICC/IF analysis of MCF7 cells using GTX57579 ARF1 antibody. Blue: DAPI Green: Primary antibody Dilution: 1:100
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REFERENCE
There are currently no references for ARF1 antibody [1B3] (GTX57579). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ARF1 antibody [1B3] (GTX57579). Be the first to share your experience with this product.