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ARF1 antibody [GT1269]

Recombinant antibody
Anti-ARF1 antibody [GT1269] used in Western Blot (WB). GTX03181
Anti-ARF1 antibody [GT1269] used in Western Blot (WB). GTX03181
Anti-ARF1 antibody [GT1269] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03181
Anti-ARF1 antibody [GT1269] used in Western Blot (WB). GTX03181

Cat. No. GTX03181

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1269

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($439)
See all ARF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human ARF1.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ADP ribosylation factor 1 , PVNH8

Cellular Localization

Golgi apparatus , Membrane

Background

ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ARF1 antibody [GT1269] used in Western Blot (WB). GTX03181

GTX03181 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ARF1 antibody [GT1269] (GTX03181) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ARF1 antibody [GT1269] used in Western Blot (WB). GTX03181

GTX03181 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ARF1 antibody [GT1269] (GTX03181) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ARF1 antibody [GT1269] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03181

GTX03181 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX03181 ARF1 antibody [GT1269].
Blue : DAPI for nuclear staining
Dilution : 1:100

Anti-ARF1 antibody [GT1269] used in Western Blot (WB). GTX03181

GTX03181 WB Image

WB analysis of various samples using GTX03181 ARF1 antibody [GT1269].
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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Package List Price ($)
$ 439