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ARF3 antibody

Anti-ARF3 antibody used in Western Blot (WB). GTX104782
Anti-ARF3 antibody used in IHC (Paraffin sections) (IHC-P). GTX104782
Anti-ARF3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104782
Anti-ARF3 antibody used in IHC (Paraffin sections) (IHC-P). GTX104782

Cat No. GTX104782

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Rat, Sheep, Bovine, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human ARF3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ADP ribosylation factor 3

Cellular Localization

Golgi apparatus

Background

ADP-ribosylation factor 3 (ARF3) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6) and members of each class share a common gene organization. The ARF3 gene contains five exons and four introns. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-ARF3 antibody used in Western Blot (WB). GTX104782

GTX104782 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
ADP-ribosylation factor 3 antibody
GTX104782 diluted at 1:1000

Anti-ARF3 antibody used in IHC (Paraffin sections) (IHC-P). GTX104782

GTX104782 IHC-P Image

ARF3 antibody detects ARF3 protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
ARF3 antibody (GTX104782) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ARF3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104782

GTX104782 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using ARF3(GTX104782) antibody at 1:500 dilution.

Anti-ARF3 antibody used in IHC (Paraffin sections) (IHC-P). GTX104782

GTX104782 IHC-P Image

ARF3 antibody detects ARF3 protein at cytosol on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
ARF3 antibody (GTX104782) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169