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ARF5 antibody

Anti-ARF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX104783
Anti-ARF5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104783
Anti-ARF5 antibody used in Western Blot (WB). GTX104783

Cat. No. GTX104783

2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ARF5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Zebrafish, Sheep, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human ARF5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240471

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ADP ribosylation factor 5

Cellular Localization

Golgi apparatus

Background

ADP-ribosylation factor 5 (ARF5) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. The ARF5 gene spans approximately 3.2kb of genomic DNA and contains six exons and five introns. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ARF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX104783

GTX104783 IHC-P Image

ARF5 antibody detects ARF5 protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ARF5 antibody (GTX104783) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ARF5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104783

GTX104783 ICC/IF Image

ARF5 antibody detects ARF5 protein at Golgi apparatus and cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ARF5 stained by ARF5 antibody (GTX104783) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-ARF5 antibody used in Western Blot (WB). GTX104783

GTX104783 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ARF5 antibody (GTX104783) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for ARF5 antibody (GTX104783). Be the first to share your publications with this product.
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REVIEW

ARF5 antibody Cat. No. GTX104783

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-ARF5 antibody used in Western Blot (WB). GTX104783
Date : Anonymous submitted on 05-Mar-2024
Score :
Application Tested : WB
Sample Species : Hu
Sample : GIST-T1 cells
Blocking : 5% skimmed milk-TBS-T, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Anti-ARF5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104783
Date : Anonymous submitted on 05-Mar-2024
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : GIST-T1 cells
Blocking : 3% BSA, 0.1% saponin, PBS, 25°C, 0.5Hr
Fixation : 4% PFA, 25°C, 0.33Hr
Permeabilization /Antigen retrieval : 0.1% saponin in PBS
Primary Antibody : 1:250, 25°C, 1Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169