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ARF5 antibody

Anti-ARF5 antibody used in Western Blot (WB). GTX119329
Anti-ARF5 antibody used in Western Blot (WB). GTX119329
Anti-ARF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX119329

Cat. No. GTX119329

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ARF5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

Raji , Mouse brain

Predict Reactivity

Rat, Zebrafish, Sheep, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ARF5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617483

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ADP ribosylation factor 5

Cellular Localization

Golgi apparatus

Background

ADP-ribosylation factor 5 (ARF5) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. The ARF5 gene spans approximately 3.2kb of genomic DNA and contains six exons and five introns. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ARF5 antibody used in Western Blot (WB). GTX119329

GTX119329 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX119329 diluted at 1:1000

Anti-ARF5 antibody used in Western Blot (WB). GTX119329

GTX119329 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
GTX119329 diluted at 1:1000

Anti-ARF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX119329

GTX119329 IHC-P Image

ARF5 antibody detects ARF5 protein at cytoplasm in respiratory epithelium of mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
ARF5 antibody (GTX119329) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169