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ARF6 antibody [N1C3]

KO/KD
Anti-ARF6 antibody [N1C3] used in Western Blot (WB). GTX112872
Anti-ARF6 antibody [N1C3] used in Western Blot (WB). GTX112872
Anti-ARF6 antibody [N1C3] used in Western Blot (WB). GTX112872

Cat. No. GTX112872

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ARF6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

MCF-7 , MDA-MB-231 , mouse brain , rat brain

Predict Reactivity

Sheep, Drosophila, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.89 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ARF6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11174316

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ADP ribosylation factor 6

Cellular Localization

Golgi apparatus

Background

This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ARF6 antibody [N1C3] used in Western Blot (WB). GTX112872

GTX112872 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ARF6 antibody (GTX112872) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ARF6 antibody [N1C3] used in Western Blot (WB). GTX112872

GTX112872 WB Image

ARF6 antibody [N1C3] detects ARF6 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12 % SDS-PAGE, and blotted with ARF6 antibody [N1C3] (GTX112872) diluted by 1:1000

Anti-ARF6 antibody [N1C3] used in Western Blot (WB). GTX112872

GTX112872 WB Image

KO/KD

The data was published in the journal Elife in 2019.PMID: 30801249

REFERENCE

Application Reference

Rivera-Serrano EE et al. Elife 2019; 8 Cellular entry and uncoating of naked and quasi-enveloped human hepatoviruses.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169