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ARF6 antibody

Anti-ARF6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54540
Anti-ARF6 antibody used in Western Blot (WB). GTX54540
Anti-ARF6 antibody used in IHC (Paraffin sections) (IHC-P). GTX54540
Anti-ARF6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54540

Cat. No. GTX54540

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Primate
Package
100 μg ($399)
See all ARF6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:20-1:100
IHC-P 1:20-100
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 30% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

C-terminal human recombinant protein fragment

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ADP ribosylation factor 6

Cellular Localization

Cytoplasm, cytosol,Cell membrane,Endosome membrane

Background

This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ARF6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54540

GTX54540 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX54540 ARF6 antibody. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-ARF6 antibody used in Western Blot (WB). GTX54540

GTX54540 WB Image

WB analysis of 50 ug of various whole cell lysate using GTX54540 ARF6 antibody.
Dilution : 1:1000

Anti-ARF6 antibody used in IHC (Paraffin sections) (IHC-P). GTX54540

GTX54540 IHC-P Image

IHC-P analysis of human heart tissue using GTX54540 ARF6 antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Anti-ARF6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54540

GTX54540 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX54540 ARF6 antibody.
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399