ICC/IF analysis of HeLa cells using GTX54540 ARF6 antibody.
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

IHC-P analysis of human heart tissue using GTX54540 ARF6 antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

WB analysis of 50 ug of various whole cell lysate using GTX54540 ARF6 antibody.
Dilution : 1:1000

ICC/IF analysis of MCF-7 cells using GTX54540 ARF6 antibody. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC