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ARFGAP1 antibody

Anti-ARFGAP1 antibody used in Western Blot (WB). GTX87479
Anti-ARFGAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87479

Cat. No. GTX87479

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Monkey
Package
100 μg ($399)
See all ARFGAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
ICC/IF 1:100~1:500
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human ARFGAP1 (171-220).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ADP ribosylation factor GTPase activating protein 1 , ARF1GAP , ARFGAP1 , HRIHFB2281

Cellular Localization

Cytoplasm,Golgi apparatus

Background

The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-ARFGAP1 antibody used in Western Blot (WB). GTX87479

GTX87479 WB Image

WB analysis of COS7 cell lysates using GTX87479 ARFGAP1 antibody. The lane on the right is blocked with the synthesized peptide.

Anti-ARFGAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87479

GTX87479 ICC/IF Image

ICC/IF analysis of MCF7 cells using GTX87479 ARFGAP1 antibody. The picture on the right is blocked with the synthesized peptide.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399