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ARFGAP3 antibody, C-term

Anti-ARFGAP3 antibody, C-term used in Western Blot (WB). GTX89988

Cat No. GTX89988

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Mouse
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89988-PEP).

Product Note

This antibody is expected to recognize both reported isoforms (NP_055385.3; NP_001135765.1).

Predict Reactivity

Human, Rat, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-NGVVTSIQDRYGS, from the C Terminus of the protein sequence according to NP_055385.3; NP_001135765.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ADP ribosylation factor GTPase activating protein 3 , ARFGAP1

Cellular Localization

Cytoplasm , Golgi apparatus membrane

Background

The protein encoded by this gene is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene. [provided by RefSeq, Nov 2008]

Database

DATA IMAGES

Anti-ARFGAP3 antibody, C-term used in Western Blot (WB). GTX89988

GTX89988 WB Image

WB analysis of mouse brain extracts using GTX89988 ARFGAP3 antibody, C-term.
Dilution : 2μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

There are currently no references for ARFGAP3 antibody, C-term (GTX89988). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for ARFGAP3 antibody, C-term (GTX89988). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf
Package List Price ($)
$ 369