APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-3μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89988-PEP).
Product Note
This antibody is expected to recognize both reported isoforms (NP_055385.3; NP_001135765.1).
Predict Reactivity
Human, Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-NGVVTSIQDRYGS, from the C Terminus of the protein sequence according to NP_055385.3; NP_001135765.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ADP ribosylation factor GTPase activating protein 3 , ARFGAP1
Cellular Localization
Cytoplasm , Golgi apparatus membrane
Background
The protein encoded by this gene is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene. [provided by RefSeq, Nov 2008]
Database
DATA IMAGES
|
GTX89988 WB Image
WB analysis of mouse brain extracts using GTX89988 ARFGAP3 antibody, C-term.
Dilution : 2μg/ml
Loading : 35μg protein in RIPA buffer
|
REFERENCE
There are currently no references for ARFGAP3 antibody, C-term (GTX89988). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ARFGAP3 antibody, C-term (GTX89988). Be the first to share your experience with this product.
