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ARFGAP3 antibody [C3], C-term

Anti-ARFGAP3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107999
Anti-ARFGAP3 antibody [C3], C-term used in Western Blot (WB). GTX107999

Cat. No. GTX107999

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ARFGAP3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ARFGAP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240472

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ADP ribosylation factor GTPase activating protein 3 , ARFGAP1

Cellular Localization

Cytoplasm , Golgi apparatus membrane

Background

The protein encoded by this gene is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene. [provided by RefSeq]

Database

DATA IMAGES

Anti-ARFGAP3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107999

GTX107999 ICC/IF Image

ARFGAP3 antibody [C3], C-term detects ARFGAP3 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in -20ºC 100% MeOH for 5 min.
Green: ARFGAP3 protein stained by ARFGAP3 antibody [C3], C-term (GTX107999) diluted at 1:500.
Blue: Hoechst 33343 staining.
Anti-ARFGAP3 antibody [C3], C-term used in Western Blot (WB). GTX107999

GTX107999 WB Image

Sample(30 μg of whole cell lysate)
A:293T
7.5% SDS PAGE
GTX107999 diluted at 1:500

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169