GeneTex
  • Country / Location Selection

United States (US)

ARFGAP3 antibody [N3C3]

Anti-ARFGAP3 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX115818
Anti-ARFGAP3 antibody [N3C3] used in Western Blot (WB). GTX115818

Cat No. GTX115818

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

NT2D1 , IMR32 , U87-MG

Predict Reactivity

Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ARFGAP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10720138

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ADP ribosylation factor GTPase activating protein 3 , ARFGAP1

Cellular Localization

Cytoplasm , Golgi apparatus membrane

Background

The protein encoded by this gene is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene. [provided by RefSeq]

Database

DATA IMAGES

Anti-ARFGAP3 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX115818

GTX115818 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using ARFGAP3(GTX115818) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ARFGAP3 antibody [N3C3] used in Western Blot (WB). GTX115818

GTX115818 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
7.5% SDS PAGE
GTX115818 diluted at 1:1000

REFERENCE

There are currently no references for ARFGAP3 antibody [N3C3] (GTX115818). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for ARFGAP3 antibody [N3C3] (GTX115818). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169