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ARFGEF2 antibody

Anti-ARFGEF2 antibody used in Western Blot (WB). GTX87126
Anti-ARFGEF2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87126

Cat. No. GTX87126

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μg ($399)
See all ARFGEF2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
ICC/IF 1:100~1:500
Not tested in other applications.

Calculated MW

202 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human ARFGEF2 (1491-1540).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ADP ribosylation factor guanine nucleotide exchange factor 2 , ARFGEF2 , BIG2 , PVNH2 , dJ1164I10.1

Cellular Localization

Cytoplasm,Membrane,Golgi apparatus,Cytoplasm,Perinuclear region,Golgi apparatus,trans-Golgi network,Endosome,Cytoplasm,Cytoskeleton,Microtubule organizing center,Centrosome,Cell projection,Dendrite,Cytoplasmic vesicle,Cell junction,Synapse,Cytoplasm,Cytoskeleton

Background

ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP and is involved in Golgi transport. It contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ARFGEF2 antibody used in Western Blot (WB). GTX87126

GTX87126 WB Image

WB analysis of A549 cell lysates using GTX87126 ARFGEF2 antibody. The lane on the right is blocked with the synthesized peptide.

Anti-ARFGEF2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87126

GTX87126 ICC/IF Image

ICC/IF analysis of A549 cells using GTX87126 ARFGEF2 antibody. The picture on the right is blocked with the synthesized peptide.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399