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ARG2 antibody [GT11111]

KO/KD
Anti-ARG2 antibody [GT11111] used in Western Blot (WB). GTX634415
Anti-ARG2 antibody [GT11111] used in IHC (Paraffin sections) (IHC-P). GTX634415
Anti-ARG2 antibody [GT11111] used in Western Blot (WB). GTX634415
Anti-ARG2 antibody [GT11111] used in IHC (Paraffin sections) (IHC-P). GTX634415

Cat. No. GTX634415

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT11111

Isotype

 IgG1

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ARG2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

mouse kidney , 293T

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ARG2. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888448

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

arginase 2

Cellular Localization

Mitochondrion

Background

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exists (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type II isoform encoded by this gene, is located in the mitochondria and expressed in extra-hepatic tissues, especially kidney. The physiologic role of this isoform is poorly understood; it is thought to play a role in nitric oxide and polyamine metabolism. Transcript variants of the type II gene resulting from the use of alternative polyadenylation sites have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ARG2 antibody [GT11111] used in Western Blot (WB). GTX634415

GTX634415 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ARG2 antibody [GT11111] (GTX634415) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ARG2 antibody [GT11111] used in IHC (Paraffin sections) (IHC-P). GTX634415

GTX634415 IHC-P Image

ARG2 antibody [GT11111] detects ARG2 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ARG2 stained by ARG2 antibody [GT11111] (GTX634415) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ARG2 antibody [GT11111] used in Western Blot (WB). GTX634415

GTX634415 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ARG2 antibody [GT11111] (GTX634415) diluted at 1:1000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ARG2 antibody [GT11111] used in IHC (Paraffin sections) (IHC-P). GTX634415

GTX634415 IHC-P Image

ARG2 antibody [GT11111] detects ARG2 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
ARG2 stained by ARG2 antibody [GT11111] (GTX634415) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations