GeneTex
United States (US)

ARG2 antibody

Orthogonal
GTX104036_42228_20160127_IHC-P_R_19011506_501.jpg
GTX104036_42613_20161205_IHC-P_M_19011506_682.jpg
GTX104036_42613_20161013_WB_19011506_652.jpg
GTX104036_39995_IHC_19011506_173.jpg
GTX104036_39995_WB_M_kidney_19011506_712.jpg
GTX104036_42228_20151126_WB_Fraction_19011506_942.jpg

Cat No. GTX104036

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

293T , NT2D1 , 293T cytoplasm extract , mouse kidney

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.32 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ARG2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Arginase 2,Arg2

Cellular Localization

Mitochondrion

Background

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exists (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type II isoform encoded by this gene, is located in the mitochondria and expressed in extra-hepatic tissues, especially kidney. The physiologic role of this isoform is poorly understood; it is thought to play a role in nitric oxide and polyamine metabolism. Transcript variants of the type II gene resulting from the use of alternative polyadenylation sites have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX104036_42228_20160127_IHC-P_R_19011506_501.jpg

GTX104036 IHC-P Image

ARG2 antibody detects ARG2 protein at mitochondria in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
ARG2 antibody (GTX104036) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX104036_42613_20161205_IHC-P_M_19011506_682.jpg

GTX104036 IHC-P Image

ARG2 antibody detects ARG2 protein at cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
ARG2 antibody (GTX104036) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX104036_42613_20161013_WB_19011506_652.jpg

GTX104036 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with ARG2 antibody (GTX104036) diluted at 1:1000.

GTX104036_39995_IHC_19011506_173.jpg

GTX104036 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Arginase II(GTX104036) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104036_39995_WB_M_kidney_19011506_712.jpg

GTX104036 WB Image

Arginase II antibody detects ARG2 protein by western blot analysis.
A. 50 µg mouse kidney lysate/extract
10% SDS-PAGE
Arginase II antibody (GTX104036) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104036_42228_20151126_WB_Fraction_19011506_942.jpg

GTX104036 WB Image

Orthogonal

293T whole cell and cytoplasm extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ARG2 antibody (GTX104036) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf