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ARG2 antibody

Orthogonal
Anti-ARG2 antibody used in Western Blot (WB). GTX104036
Anti-ARG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX104036
Anti-ARG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX104036
Anti-ARG2 antibody used in Western Blot (WB). GTX104036
Anti-ARG2 antibody used in Western Blot (WB). GTX104036
Anti-ARG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX104036
Anti-ARG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX104036

Cat. No. GTX104036

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ARG2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

mouse kidney , 293T cytoplasm extract , NT2D1

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.32 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ARG2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036262

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

arginase 2

Cellular Localization

Mitochondrion

Background

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exists (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type II isoform encoded by this gene, is located in the mitochondria and expressed in extra-hepatic tissues, especially kidney. The physiologic role of this isoform is poorly understood; it is thought to play a role in nitric oxide and polyamine metabolism. Transcript variants of the type II gene resulting from the use of alternative polyadenylation sites have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ARG2 antibody used in Western Blot (WB). GTX104036

GTX104036 WB Image

Arginase II antibody detects ARG2 protein by western blot analysis.
A. 50 μg mouse kidney lysate/extract
10% SDS-PAGE
Arginase II antibody (GTX104036) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ARG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX104036

GTX104036 IHC-P Image

ARG2 antibody detects ARG2 protein at cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
ARG2 antibody (GTX104036) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ARG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX104036

GTX104036 IHC-P Image

ARG2 antibody detects ARG2 protein at mitochondria in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
ARG2 antibody (GTX104036) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ARG2 antibody used in Western Blot (WB). GTX104036

GTX104036 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ARG2 antibody (GTX104036) diluted at 1:1000.

Anti-ARG2 antibody used in Western Blot (WB). GTX104036

GTX104036 WB Image

Orthogonal

293T whole cell and cytoplasm extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ARG2 antibody (GTX104036) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ARG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX104036

GTX104036 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Arginase II(GTX104036) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ARG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX104036

GTX104036 IHC-P Image

The data was published in the journal Neurotherapeutics in 2018. PMID: 30288668

REFERENCE

Application Reference

Polis B et al. Neural Regen Res 2019; 14 (9) : 1562-1572 L-Norvaline
Sato T et al. Arterioscler Thromb Vasc Biol 2019; 39 (9) : 1802-1816 Preferential Incorporation of Administered Eicosapentaenoic Acid Into Thin-Cap Atherosclerotic Plaques.
Application : IHC-Fr
Reactivity : Mouse
Polis B et al. Neurotherapeutics 2018; 15 (4) : 1036-1054 L-Norvaline Reverses Cognitive Decline and Synaptic Loss in a Murine Model of Alzheimer's Disease.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169