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ARG2 antibody

KO/KD Orthogonal
Anti-ARG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX118048
Anti-ARG2 antibody used in Western Blot (WB). GTX118048
Anti-ARG2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118048
Anti-ARG2 antibody used in Western Blot (WB). GTX118048
Anti-ARG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX118048
Anti-ARG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX118048
Anti-ARG2 antibody used in Western Blot (WB). GTX118048

Cat. No. GTX118048

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ARG2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ARG2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163078

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

arginase 2

Cellular Localization

Mitochondrion

Background

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exists (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type II isoform encoded by this gene, is located in the mitochondria and expressed in extra-hepatic tissues, especially kidney. The physiologic role of this isoform is poorly understood; it is thought to play a role in nitric oxide and polyamine metabolism. Transcript variants of the type II gene resulting from the use of alternative polyadenylation sites have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ARG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX118048

GTX118048 IHC-P Image

ARG2 antibody detects ARG2 protein at mitochondria on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
ARG2 antibody (GTX118048) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ARG2 antibody used in Western Blot (WB). GTX118048

GTX118048 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ARG2 antibody (GTX118048) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ARG2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118048

GTX118048 ICC/IF Image

ARG2 antibody detects ARG2 protein at mitochondria by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: ARG2 protein stained by ARG2 antibody (GTX118048) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-ARG2 antibody used in Western Blot (WB). GTX118048

GTX118048 WB Image

Orthogonal

293T whole cell and cytoplasm extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ARG2 antibody (GTX118048) diluted at 1:1000.

Anti-ARG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX118048

GTX118048 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using ARG2(GTX118048) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ARG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX118048

GTX118048 IHC-P Image

ARG2 antibody detects ARG2 protein at mitochondria on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ARG2 antibody (GTX118048) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ARG2 antibody used in Western Blot (WB). GTX118048

GTX118048 WB Image

The data was published in the journal Sci Rep in 2017.PMID: 28211494

REFERENCE

Application Reference

Troiano A et al. EMBO Rep 2020; 21 (6) : e48942 ZSCAN4+ mouse embryonic stem cells have an oxidative and flexible metabolic profile.
Application : IHC-P
Reactivity : Mouse
Royo F et al. Sci Rep 2017; 7 : 42798 Hepatocyte-secreted extracellular vesicles modify blood metabolome and endothelial function by an arginase-dependent mechanism.
Application : WB
Reactivity : Rat
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169