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ARGBP2 antibody, N-term

Anti-ARGBP2 antibody, N-term used in Western Blot (WB). GTX81600
Anti-ARGBP2 antibody, N-term used in Flow cytometry (FACS). GTX81600
Anti-ARGBP2 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX81600
Anti-ARGBP2 antibody, N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX81600
Anti-ARGBP2 antibody, N-term used in Western Blot (WB). GTX81600

Cat. No. GTX81600

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
400 μl ($399)
See all ARGBP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:10-1:50
IHC-P 1:10-1:50
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

124 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 175-204 amino acids from the N-terminal region of human ARGBP2.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

RRID

AB_11163340

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sorbin and SH3 domain containing 2 , ARGBP2 , PRO0618

Cellular Localization

Apical cell membrane

Background

Arg and c-Abl represent the mammalian members of the Abelson family of non-receptor protein-tyrosine kinases. They interact with the Arg/Abl binding proteins via the SH3 domains present in the carboxy end of the latter group of proteins. This gene encodes the sorbin and SH3 domain containing 2 protein. It has three C-terminal SH3 domains and an N-terminal sorbin homology (SoHo) domain that interacts with lipid raft proteins. The subcellular localization of this protein in epithelial and cardiac muscle cells suggests that it functions as an adapter protein to assemble signaling complexes in stress fibers, and that it is a potential link between Abl family kinases and the actin cytoskeleton. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ARGBP2 antibody, N-term used in Western Blot (WB). GTX81600

GTX81600 WB Image

WB analysis of MDA-MB231 cell lysate (35ug/lane) using GTX81600 ARGBP2 antibody, N-term.

Anti-ARGBP2 antibody, N-term used in Flow cytometry (FACS). GTX81600

GTX81600 FACS Image

FACS analysis of MDA-231 cells using GTX81600 ARGBP2 antibody, N-term.
Top histogram : negative control
Bottom histogram : MDA-231 cells

Anti-ARGBP2 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX81600

GTX81600 IHC-P Image

IHC-P analysis of human colon carcinoma using GTX81600 ARGBP2 antibody, N-term.

Anti-ARGBP2 antibody, N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX81600

GTX81600 ICC/IF Image

ICC/IF analysis of U251 cells using GTX81600 ARGBP2 antibody, N-term.
Green : ARGBP2
Red : Actin filament
Fixation : 4% paraformaldehyde (PFA) (20 min)
Permeabilization : Triton X-100 (0.1%, 10 min)
Dilution : 1:25

Anti-ARGBP2 antibody, N-term used in Western Blot (WB). GTX81600

GTX81600 WB Image

The data was published in the journal PeerJ in 2018.PMID: 30065891

REFERENCE

Application Reference

Roberts MD et al. PeerJ 2018; 6 : e5338 Skeletal muscle mitochondrial volume and myozenin-1 protein differences exist between high versus low anabolic responders to resistance training.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399