APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
10μg/ml |
Note :
ICC/IF
Strong expression of the protein seen in the cytoplasm of U2OS and A431 cells.
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Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX25082-PEP).
Predict Reactivity
Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence DALKSAGRALIRS-C, from the N Terminus of the protein sequence according to NP_056442.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
low density lipoprotein receptor adaptor protein 1 , ARH , ARH1 , ARH2 , FHCB1 , FHCB2
Cellular Localization
Cytoplasm
Background
The protein encoded by this gene is a cytosolic protein which contains a phosphotyrosine binding (PTD) domain. The PTD domain has been found to interact with the cytoplasmic tail of the LDL receptor. Mutations in this gene lead to LDL receptor malfunction and cause the disorder autosomal recessive hypercholesterolaemia. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX25082 ICC/IF Image
ICC/IF analysis of PFA fixed U2OS cells using GTX25082 ARH antibody, N-term. Green : Primary antibody Blue : DAPI Negative control : Unimmunized goat IgG Permeabilization : 0.15% Triton Dilution : 10μg/ml
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GTX25082 ICC/IF Image
ICC/IF analysis of PFA fixed A431 cells using GTX25082 ARH antibody, N-term. Green : Primary antibody Blue : DAPI Negative control : Unimmunized goat IgG Permeabilization : 0.15% Triton Dilution : 10μg/ml
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REFERENCE
There are currently no references for ARH antibody, N-term (GTX25082). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ARH antibody, N-term (GTX25082). Be the first to share your experience with this product.