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ARHGAP1 antibody [N1N3]

Anti-ARHGAP1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101472
Anti-ARHGAP1 antibody [N1N3] used in Western Blot (WB). GTX101472
Anti-ARHGAP1 antibody [N1N3] used in Western Blot (WB). GTX101472

Cat No. GTX101472

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

ARHGAP1 tansfected 293T cell

Predict Reactivity

Mouse, Rat, Bovine, Dog, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ARHGAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Rho GTPase activating protein 1,CDC42GAP,RHOGAP,RHOGAP1,p50rhoGAP

Cellular Localization

Cytoplasm

Background

This gene encodes a member of a large family of proteins that activate Rho-type guanosine triphosphate (GTP) metabolizing enzymes. The encoded protein contains a SRC homology 3 domain and interacts with Bcl-2-associated protein family members. [provided by RefSeq, Aug 2012]

Database

  • Gene ID: 392 ARHGAP1
  • UniProt: Q07960 ARHGAP1
DATA IMAGES
Anti-ARHGAP1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101472

GTX101472 ICC/IF Image

ARHGAP1 antibody [N1N3] detects ARHGAP1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ARHGAP1 protein stained by ARHGAP1 antibody [N1N3] (GTX101472) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-ARHGAP1 antibody [N1N3] used in Western Blot (WB). GTX101472

GTX101472 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ARHGAP1 antibody [N1N3] (GTX101472) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ARHGAP1 antibody [N1N3] used in Western Blot (WB). GTX101472

GTX101472 WB Image

ARHGAP1 antibody detects ARHGAP1 protein by Western blot analysis. Non-transfected (-) and ARHGAP1-transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ARHGAP1 antibody (GTX101472) diluted at 1:1000.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169