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ARHGAP1 antibody [N1N3]

Overexpression
Anti-ARHGAP1 antibody [N1N3] used in Western Blot (WB). GTX101472
Anti-ARHGAP1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101472
Anti-ARHGAP1 antibody [N1N3] used in Western Blot (WB). GTX101472

Cat. No. GTX101472

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ARHGAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

human ARHGAP1-transfected 293T , 293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ARHGAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949680

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Rho GTPase activating protein 1 , CDC42GAP , RHOGAP , RHOGAP1 , p50rhoGAP

Cellular Localization

Cytoplasm

Background

This gene encodes a member of a large family of proteins that activate Rho-type guanosine triphosphate (GTP) metabolizing enzymes. The encoded protein contains a SRC homology 3 domain and interacts with Bcl-2-associated protein family members. [provided by RefSeq, Aug 2012]

Database

  • Gene ID: 392 ARHGAP1
  • UniProt: Q07960 ARHGAP1

Research Area

DATA IMAGES

Anti-ARHGAP1 antibody [N1N3] used in Western Blot (WB). GTX101472

GTX101472 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ARHGAP1 antibody [N1N3] (GTX101472) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ARHGAP1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101472

GTX101472 ICC/IF Image

ARHGAP1 antibody [N1N3] detects ARHGAP1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ARHGAP1 protein stained by ARHGAP1 antibody [N1N3] (GTX101472) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-ARHGAP1 antibody [N1N3] used in Western Blot (WB). GTX101472

GTX101472 WB Image

Overexpression

ARHGAP1 antibody detects ARHGAP1 protein by Western blot analysis. Non-transfected (-) and ARHGAP1-transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ARHGAP1 antibody (GTX101472) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169