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ARHGAP1 antibody [N1N3]

Anti-ARHGAP1 antibody [N1N3] used in Western Blot (WB). GTX101472
Anti-ARHGAP1 antibody [N1N3] used in Western Blot (WB). GTX101472
Anti-ARHGAP1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101472

Cat No. GTX101472

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

ARHGAP1 tansfected 293T cell

Predict Reactivity

Mouse, Rat, Bovine, Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ARHGAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949680

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Rho GTPase activating protein 1 , CDC42GAP , RHOGAP , RHOGAP1 , p50rhoGAP

Cellular Localization

Cytoplasm

Background

This gene encodes a member of a large family of proteins that activate Rho-type guanosine triphosphate (GTP) metabolizing enzymes. The encoded protein contains a SRC homology 3 domain and interacts with Bcl-2-associated protein family members. [provided by RefSeq, Aug 2012]

Database

  • Gene ID: 392 ARHGAP1
  • UniProt: Q07960 ARHGAP1

Research Area

DATA IMAGES

Anti-ARHGAP1 antibody [N1N3] used in Western Blot (WB). GTX101472

GTX101472 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ARHGAP1 antibody [N1N3] (GTX101472) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ARHGAP1 antibody [N1N3] used in Western Blot (WB). GTX101472

GTX101472 WB Image

ARHGAP1 antibody detects ARHGAP1 protein by Western blot analysis. Non-transfected (-) and ARHGAP1-transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ARHGAP1 antibody (GTX101472) diluted at 1:1000.

Anti-ARHGAP1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101472

GTX101472 ICC/IF Image

ARHGAP1 antibody [N1N3] detects ARHGAP1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ARHGAP1 protein stained by ARHGAP1 antibody [N1N3] (GTX101472) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

There are currently no references for ARHGAP1 antibody [N1N3] (GTX101472). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169