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ARHGAP1 antibody [N3C3]

Anti-ARHGAP1 antibody [N3C3] used in Western Blot (WB). GTX101471
Anti-ARHGAP1 antibody [N3C3] used in Western Blot (WB). GTX101471
Anti-ARHGAP1 antibody [N3C3] used in Western Blot (WB). GTX101471
Anti-ARHGAP1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101471

Cat No. GTX101471

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Mouse brain , ARHGAP1-transfected 293T

Predict Reactivity

Rat, Bovine, Dog, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ARHGAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Rho GTPase activating protein 1,CDC42GAP,RHOGAP,RHOGAP1,p50rhoGAP

Cellular Localization

Cytoplasm

Background

GTPase activator for the Rho, Rac and Cdc42 proteins, converting them to the putatively inactive GDP-bound state. Cdc42 seems to be the preferred substrate.

Database

  • Gene ID: 392 ARHGAP1
  • UniProt: Q07960 ARHGAP1
DATA IMAGES
Anti-ARHGAP1 antibody [N3C3] used in Western Blot (WB). GTX101471

GTX101471 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ARHGAP1 antibody [N3C3] (GTX101471) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ARHGAP1 antibody [N3C3] used in Western Blot (WB). GTX101471

GTX101471 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX101471 diluted at 1:1000

Anti-ARHGAP1 antibody [N3C3] used in Western Blot (WB). GTX101471

GTX101471 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ARHGAP1 antibody [N3C3] (GTX101471) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ARHGAP1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101471

GTX101471 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung SCC, using ARHGAP1(GTX101471) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
There are currently no references for ARHGAP1 antibody [N3C3] (GTX101471). Be the first to share your publications with this product.
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169