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ARHGAP1 antibody [N3C3]

Overexpression
Anti-ARHGAP1 antibody [N3C3] used in Western Blot (WB). GTX101471
Anti-ARHGAP1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101471
Anti-ARHGAP1 antibody [N3C3] used in Western Blot (WB). GTX101471
Anti-ARHGAP1 antibody [N3C3] used in Western Blot (WB). GTX101471

Cat. No. GTX101471

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ARHGAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

Mouse brain , ARHGAP1-transfected 293T , 293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Bovine, Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ARHGAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949681

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Rho GTPase activating protein 1 , CDC42GAP , RHOGAP , RHOGAP1 , p50rhoGAP

Cellular Localization

Cytoplasm

Background

GTPase activator for the Rho, Rac and Cdc42 proteins, converting them to the putatively inactive GDP-bound state. Cdc42 seems to be the preferred substrate.

Database

  • Gene ID: 392 ARHGAP1
  • UniProt: Q07960 ARHGAP1

Research Area

DATA IMAGES

Anti-ARHGAP1 antibody [N3C3] used in Western Blot (WB). GTX101471

GTX101471 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX101471 diluted at 1:1000

Anti-ARHGAP1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101471

GTX101471 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung SCC, using ARHGAP1(GTX101471) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ARHGAP1 antibody [N3C3] used in Western Blot (WB). GTX101471

GTX101471 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ARHGAP1 antibody [N3C3] (GTX101471) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ARHGAP1 antibody [N3C3] used in Western Blot (WB). GTX101471

GTX101471 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ARHGAP1 antibody [N3C3] (GTX101471) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169