GeneTex
United States (US)

ARHGAP17 antibody

RHG17-antibody-GTX87907-WB-1_18122102_622.jpg

Cat No. GTX87907

Host Rabbit
Clonality Polyclonal
Application WB, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
ELISA: 1:40000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

95 kDa. ( Note )

Specificity/Sensitivity

RHG17 Antibody detects endogenous levels of total ARHGAP17 protein.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human ARHGAP17.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Rho Gtpase Activating Protein 17,Mst066,Mst110,Mstp038,Mstp066,Mstp110,Nadrin,Pp367,Pp4534,Rich-1,Rich1,Wbp15,Arhgap17

Cellular Localization

Associates with membranes and concentrates at sites of cell-cell contact.

Background

Rho GTPase-activating protein involved in the maintenance of tight junction by regulating the activity of CDC42, thereby playing a central role in apical polarity of epithelial cells. Specifically acts as a GTPase activator for the CDC42 GTPase by converting it to an inactive GDP-bound state. The complex formed with AMOT acts by regulating the uptake of polarity proteins at tight junctions, possibly by deciding whether tight junction transmembrane proteins are recycled back to the plasma membrane or sent elsewhere. Participates in the Ca(2+)-dependent regulation of exocytosis, possibly by catalyzing GTPase activity of Rho family proteins and by inducing the reorganization of the cortical actin filaments. Acts as a GTPase activitor in vitro for RAC1.

Database

DATA IMAGES
RHG17-antibody-GTX87907-WB-1_18122102_622.jpg

GTX87907 WB Image

Western blot analysis of extracts from LOVO cells, using RHG17 Antibody. The lane on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339