GeneTex
United States (US)

ARHGAP22 antibody

RHG22-antibody-GTX87597-WB-1_18122102_789.jpg

Cat No. GTX87597

Host Rabbit
Clonality Polyclonal
Application WB, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Specificity/Sensitivity

RHG22 Antibody detects endogenous levels of total ARHGAP22 protein.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human ARHGAP22.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Rho-type GTPase-activating protein 22

Cellular Localization

Mainly cytoplasmic. Some fraction is nuclear.

Background

Rho GTPase-activating protein involved in the signal transduction pathway that regulates endothelial cell capillary tube formation during angiogenesis. Acts as a GTPase activator for the RAC1 by converting it to an inactive GDP-bound state. Inhibits RAC1-dependent lamellipodia formation. May also play a role in transcription regulation via its interaction with VEZF1, by regulating activity of the endothelin-1 (EDN1) promoter.
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Database

Research Area

DATA IMAGES
RHG22-antibody-GTX87597-WB-1_18122102_789.jpg

GTX87597 WB Image

Western blot analysis of extracts from K562 cells, using RHG22 Antibody. The lane on the right is treated with the synthesized peptide.