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ARHGAP25 antibody [3G1]

Overexpression
Anti-ARHGAP25 antibody [3G1] used in Western Blot (WB). GTX84863
Anti-ARHGAP25 antibody [3G1] used in IHC (Paraffin sections) (IHC-P). GTX84863
Anti-ARHGAP25 antibody [3G1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84863
Anti-ARHGAP25 antibody [3G1] used in Flow cytometry (FACS). GTX84863
Anti-ARHGAP25 antibody [3G1] used in Western Blot (WB). GTX84863
Anti-ARHGAP25 antibody [3G1] used in IHC (Paraffin sections) (IHC-P). GTX84863

Cat. No. GTX84863

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3G1

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μl ($519)
See all ARHGAP25 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:150
FACS 1:100
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.78 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human ARHGAP25 (NP_055697) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Rho GTPase activating protein 25 , HEL-S-308 , KAIA0053

Background

GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.

Database

Research Area

DATA IMAGES

Anti-ARHGAP25 antibody [3G1] used in Western Blot (WB). GTX84863

GTX84863 WB Image

WB analysis of various cell lines using GTX84863 ARHGAP25 antibody [3G1].
Loading : 35 ug per lane

Anti-ARHGAP25 antibody [3G1] used in IHC (Paraffin sections) (IHC-P). GTX84863

GTX84863 IHC-P Image

IHC-P analysis of human lymph node tissue using GTX84863 ARHGAP25 antibody [3G1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-ARHGAP25 antibody [3G1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84863

GTX84863 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with ARHGAP25 plasmid using GTX84863 ARHGAP25 antibody [3G1].

Anti-ARHGAP25 antibody [3G1] used in Flow cytometry (FACS). GTX84863

GTX84863 FACS Image

FACS analysis of HEK293T cells transfected with either ARHGAP25 plasmid(Red) or empty vector control plasmid(Blue) using GTX84863 ARHGAP25 antibody [3G1].

Anti-ARHGAP25 antibody [3G1] used in Western Blot (WB). GTX84863

GTX84863 WB Image

Overexpression

WB analysis of HEK293T cells transfected with ARHGAP25 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84863 ARHGAP25 antibody [3G1].
Loading : 5 ug per lane

Anti-ARHGAP25 antibody [3G1] used in IHC (Paraffin sections) (IHC-P). GTX84863

GTX84863 IHC-P Image

IHC-P analysis of human lymphoma tissue using GTX84863 ARHGAP25 antibody [3G1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519