GeneTex
United States (US)

ARHGAP4 antibody [N1N2], N-term

GTX103253_39848_IHC_19011506_381.jpg
GTX103253_WB_19011506_649.jpg
GTX103253_IFA_19011506_973.jpg

Cat No. GTX103253

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

105 kDa. ( Note )

Positive Control

Jurkat , NT2D1

Predict Reactivity

Mouse, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ARHGAP4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

C1 antibody, KIAA0131 antibody, RGC1 antibody, RhoGAP4 antibody, SrGAP4 antibody, p115 antibody, ARHGAP4 antibody, rho-type GTPase-activating protein 4 antibody, Rho-GAP hematopoietic protein C1 antibody, rho GTPase-activating protein 4 antibody, Rho GTPase activating protein 4 antibody

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the rhoGAP family of proteins which play a role in the regulation of small GTP-binding proteins belonging to the RAS superfamily. The protein encoded by the orthologous gene in rat is localized to the Golgi complex and can redistribute to microtubules. The rat protein stimulates the activity of some Rho GTPases in vitro. Genomic deletions of this gene and a neighboring gene have been found in patients with nephrogenic diabetes insipidus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

DATA IMAGES
GTX103253_39848_IHC_19011506_381.jpg

GTX103253 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ARHGAP4(GTX103253) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103253_WB_19011506_649.jpg

GTX103253 WB Image

Sample (30 ug of whole cell lysate)
A: JurKat
7.5% SDS PAGE
GTX103253 diluted at 1:1000

GTX103253_IFA_19011506_973.jpg

GTX103253 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using Rho GAP4(GTX103253) antibody at 1:200 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169