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ARHGEF18 antibody, C-term

Anti-ARHGEF18 antibody, C-term used in Western Blot (WB). GTX10152

Cat No. GTX10152

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μg ($299)
See all ARHGEF18 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
ICC/IF Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

131 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX10152-PEP).

Specificity/Sensitivity

This antibody is expected to recognize both reported isoforms (NP_056133.2; NP_001124427.1).

PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-SAKEDASKEDVIFF, from the C Terminus of the protein sequence according to NP_056133.2; NP_001124427.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Rho/Rac Guanine Nucleotide Exchange Factor 18 , P114-Rhogef , Rp78 , Arhgef18

Cellular Localization

Colocalizes with actin stress fibers.

Background

Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription, cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). The protein encoded by this gene is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-ARHGEF18 antibody, C-term used in Western Blot (WB). GTX10152

GTX10152 WB Image

WB analysis of Daudi (A) and Caco-2 (B) cell lysate using GTX10152 ARHGEF18 antibody, C-term.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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Package List Price ($)
$ 299