APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-3μg/ml |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX10152-PEP).
Product Note
This antibody is expected to recognize both reported isoforms (NP_056133.2; NP_001124427.1).
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-SAKEDASKEDVIFF, from the C Terminus of the protein sequence according to NP_056133.2; NP_001124427.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Rho/Rac guanine nucleotide exchange factor 18 , P114-RhoGEF , RP78 , SA-RhoGEF , p114RhoGEF
Cellular Localization
Cytoplasm , Cytoplasm, cytoskeleton , Cell membrane , Apical cell membrane
Background
Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription, cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). The protein encoded by this gene is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2008]
Database
Research Area
DATA IMAGES
|
GTX10152 WB Image
WB analysis of Daudi (A) and Caco-2 (B) cell lysate using GTX10152 ARHGEF18 antibody, C-term.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer
|
REFERENCE
There are currently no references for ARHGEF18 antibody, C-term (GTX10152). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ARHGEF18 antibody, C-term (GTX10152). Be the first to share your experience with this product.
