APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1-3μg/ml |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX10152-PEP).
Specificity/Sensitivity
This antibody is expected to recognize both reported isoforms (NP_056133.2; NP_001124427.1).
PROPERTIES
Form
Liquid
Buffer
Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-SAKEDASKEDVIFF, from the C Terminus of the protein sequence according to NP_056133.2; NP_001124427.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Rho/Rac Guanine Nucleotide Exchange Factor 18 , P114-Rhogef , Rp78 , Arhgef18
Cellular Localization
Colocalizes with actin stress fibers.
Background
Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription, cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). The protein encoded by this gene is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2008]
Database
DATA IMAGES
|
GTX10152 WB Image
WB analysis of Daudi (A) and Caco-2 (B) cell lysate using GTX10152 ARHGEF18 antibody, C-term.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer
|
REFERENCE
There are currently no references for ARHGEF18 antibody, C-term (GTX10152). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ARHGEF18 antibody, C-term (GTX10152). Be the first to share your experience with this product.
