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ARHGEF18 antibody [GT1972]

Anti-ARHGEF18 antibody [GT1972] used in Western Blot (WB). GTX629806
Anti-ARHGEF18 antibody [GT1972] used in IHC (Paraffin sections) (IHC-P). GTX629806
Anti-ARHGEF18 antibody [GT1972] used in Western Blot (WB). GTX629806
Anti-ARHGEF18 antibody [GT1972] used in IHC (Paraffin sections) (IHC-P). GTX629806
Anti-ARHGEF18 antibody [GT1972] used in Western Blot (WB). GTX629806

Cat. No. GTX629806

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1972

Isotype

 IgG2a

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ARHGEF18 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

131 kDa. ( Note )

Positive Control

Neuro2A , GL261 , C8D30 , NIH-3T3 , C2C12 , PC-12 , Rat-2 , 293T , A431 , HeLa , HepG2

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ARHGEF18. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888157

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Rho/Rac guanine nucleotide exchange factor 18 , P114-RhoGEF , RP78 , SA-RhoGEF , p114RhoGEF

Cellular Localization

Cytoplasm

Background

Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription, cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). The protein encoded by this gene is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-ARHGEF18 antibody [GT1972] used in Western Blot (WB). GTX629806

GTX629806 WB Image

ARHGEF18 antibody [GT1972] detects ARHGEF18 protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat-2 whole cell lysate/extract
5 % SDS-PAGE
ARHGEF18 antibody [GT1972] (GTX629806) dilution: 1:1000

Anti-ARHGEF18 antibody [GT1972] used in IHC (Paraffin sections) (IHC-P). GTX629806

GTX629806 IHC-P Image

ARHGEF18 antibody [GT1972] detects ARHGEF18 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat lymph node.
ARHGEF18 stained by ARHGEF18 antibody [GT1972] (GTX629806) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ARHGEF18 antibody [GT1972] used in Western Blot (WB). GTX629806

GTX629806 WB Image

ARHGEF18 antibody [GT1972] detects ARHGEF18 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
5 % SDS-PAGE
ARHGEF18 antibody [GT1972] (GTX629806) dilution: 1:3000

Anti-ARHGEF18 antibody [GT1972] used in IHC (Paraffin sections) (IHC-P). GTX629806

GTX629806 IHC-P Image

ARHGEF18 antibody [GT1972] detects ARHGEF18 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse thymus gland.
ARHGEF18 stained by ARHGEF18 antibody [GT1972] (GTX629806) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ARHGEF18 antibody [GT1972] used in Western Blot (WB). GTX629806

GTX629806 WB Image

ARHGEF18 antibody [GT1972] detects ARHGEF18 protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg C2C12 whole cell lysate/extract
5 % SDS-PAGE
ARHGEF18 antibody [GT1972] (GTX629806) dilution: 1:1000

REFERENCE

Application Reference

Zihni C et al. Nat Cell Biol 2017; 19 (9) : 1049-1060 An apical MRCK-driven morphogenetic pathway controls epithelial polarity.
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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations