GeneTex
United States (US)

ARHGEF18 antibody

GTX102223_39820_IFA_19011506_930.jpg
GTX102223_39820_20180504_IHC-P_M_19011506_252.jpg
GTX102223_39820_20180504_IHC-P_R_19011506_948.jpg
GTX102223_WB_19011506_975.jpg

Cat No. GTX102223

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

131 kDa. ( Note )

Positive Control

HepG2
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ARHGEF18. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

KIAA0521 antibody, MGC15913 antibody, P114-RhoGEF antibody, ARHGEF18 antibody, SA-RhoGEF antibody, Rho-specific guanine nucleotide exchange factor p114 antibody, rho guanine nucleotide exchange factor 18 antibody, P114-RHO-GEF antibody, 114 kDa Rho-specific guanine nucleotide exchange factor antibody, septin-associated RhoGEF antibody, Rho/Rac guanine nucleotide exchange factor 18 antibody, p114RhoGEF antibody, Rho/Rac guanine nucleotide exchange factor (GEF) 18 antibody

Cellular Localization

Cytoplasm

Background

Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription, cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). The protein encoded by this gene is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Database

DATA IMAGES
GTX102223_39820_IFA_19011506_930.jpg

GTX102223 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using ARHGEF18(GTX102223) antibody at 1:200 dilution.

GTX102223_39820_20180504_IHC-P_M_19011506_252.jpg

GTX102223 IHC-P Image

ARHGEF18 antibody detects ARHGEF18 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse thymus gland.
ARHGEF18 stained by ARHGEF18 antibody (GTX102223) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102223_39820_20180504_IHC-P_R_19011506_948.jpg

GTX102223 IHC-P Image

ARHGEF18 antibody detects ARHGEF18 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
ARHGEF18 stained by ARHGEF18 antibody (GTX102223) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102223_WB_19011506_975.jpg

GTX102223 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX102223 diluted at 1:1000

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169