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ARHGEF3 antibody [N1C1]

Anti-ARHGEF3 antibody [N1C1] used in Western Blot (WB). GTX116114
Anti-ARHGEF3 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX116114
Anti-ARHGEF3 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116114

Cat. No. GTX116114

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ARHGEF3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

MCF-7

Predict Reactivity

Mouse, Rat, Zebrafish, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ARHGEF3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10622372

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Rho guanine nucleotide exchange factor 3 , GEF3 , STA3 , XPLN

Cellular Localization

Cytoplasm

Background

Rho-like GTPases are involved in a variety of cellular processes, and they are activated by binding GTP and inactivated by conversion of GTP to GDP by their intrinsic GTPase activity. Guanine nucleotide exchange factors (GEFs) accelerate the GTPase activity of Rho GTPases by catalyzing their release of bound GDP. This gene encodes a guanine nucleotide exchange factor, which specifically activates two members of the Rho GTPase family: RHOA and RHOB, both of which have a role in bone cell biology. It has been identified that genetic variation in this gene plays a role in the determination of bone mineral density (BMD), indicating the implication of this gene in postmenopausal osteoporosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ARHGEF3 antibody [N1C1] used in Western Blot (WB). GTX116114

GTX116114 WB Image

Sample (30 μg of whole cell lysate)
A: MCF-7
7.5% SDS PAGE
GTX116114 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ARHGEF3 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX116114

GTX116114 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using ARHGEF3(GTX116114) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ARHGEF3 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116114

GTX116114 ICC/IF Image

ARHGEF3 antibody [N1C1] detects ARHGEF3 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ARHGEF3 protein stained by ARHGEF3 antibody [N1C1] (GTX116114) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Yamamura S et al. PLoS One 2012; 7 (1) : e29722 MicroRNA-34a modulates c-Myc transcriptional complexes to suppress malignancy in human prostate cancer cells.
Yamamura S et al. Carcinogenesis 2012; 33 (2) : 294-300 MicroRNA-34a suppresses malignant transformation by targeting c-Myc transcriptional complexes in human renal cell carcinoma.
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169