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ARHGEF4 antibody, N-term

Anti-ARHGEF4 antibody, N-term used in Western Blot (WB). GTX89645
Anti-ARHGEF4 antibody, N-term used in Western Blot (WB). GTX89645

Cat No. GTX89645

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
Package
100μg ($329)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 2-4μg/ml
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89645-PEP).

Specificity/Sensitivity

This antibody is expected to recognise both reported isoforms (NP_056135.2; NP_127462.1).
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence PWEEPAGEKPSCS, from the N Terminus of the protein sequence according to NP_056135.2; NP_127462.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Rho Guanine Nucleotide Exchange Factor 4,Asef,Asef1,Gef4,Stm6,Arhgef4

Cellular Localization

Associated with membrane ruffles.

Background

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. This protein is similar to rat collybistin protein. Alternative splicing of this gene generates two transcript variants which encode different isoforms. Also there is possibility for the usage of multiple polyadenylation sites for this gene. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
Anti-ARHGEF4 antibody, N-term used in Western Blot (WB). GTX89645

GTX89645 WB Image

WB analysis of human brain lysate using GTX89645 ARHGEF4 antibody, N-term.
Dilution : 2µg/ml
Loading : 35µg protein in RIPA buffer

Anti-ARHGEF4 antibody, N-term used in Western Blot (WB). GTX89645

GTX89645 WB Image

WB analysis of HEK293 overexpressing ARHGEF4 (mock transfection in first lane) using GTX89645 ARHGEF4 antibody, N-term.

REFERENCE
There are currently no references for ARHGEF4 antibody, N-term (GTX89645).
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REVIEW
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Package List Price ($)
$ 329