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ARID1A antibody [GT3611]

Anti-ARID1A antibody [GT3611] used in IHC (Paraffin sections) (IHC-P). GTX632013
Anti-ARID1A antibody [GT3611] used in Western Blot (WB). GTX632013
Anti-ARID1A antibody [GT3611] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632013
Anti-ARID1A antibody [GT3611] used in IHC (Paraffin sections) (IHC-P). GTX632013
Anti-ARID1A antibody [GT3611] used in IHC (Paraffin sections) (IHC-P). GTX632013

Cat. No. GTX632013

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT3611

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ARID1A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

242 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.61 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ARID1A. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888276

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AT-rich interaction domain 1A , B120 , BAF250 , BAF250a , BM029 , C1orf4 , CSS2 , ELD , MRD14 , OSA1 , P270 , SMARCF1 , hELD , hOSA1

Cellular Localization

Nucleus

Background

This gene encodes a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ARID1A antibody [GT3611] used in IHC (Paraffin sections) (IHC-P). GTX632013

GTX632013 IHC-P Image

ARID1A antibody [GT3611] detects ARID1A protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
ARID1A stained by ARID1A antibody [GT3611] (GTX632013) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ARID1A antibody [GT3611] used in Western Blot (WB). GTX632013

GTX632013 WB Image

ARID1A antibody [GT3611] detects ARID1A protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ARID1A antibody [GT3611] (GTX632013) at a dilution of 1:500.

Anti-ARID1A antibody [GT3611] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632013

GTX632013 ICC/IF Image

ARID1A antibody [GT3611] detects ARID1A protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ARID1A stained by ARID1A antibody [GT3611] (GTX632013) diluted at 1:200.
Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody (GTX112141) diluted at 1:500.

Anti-ARID1A antibody [GT3611] used in IHC (Paraffin sections) (IHC-P). GTX632013

GTX632013 IHC-P Image

ARID1A antibody [GT3611] detects ARID1A protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
ARID1A stained by ARID1A antibody [GT3611] (GTX632013) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ARID1A antibody [GT3611] used in IHC (Paraffin sections) (IHC-P). GTX632013

GTX632013 IHC-P Image

ARID1A antibody [GT3611] detects ARID1A protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
ARID1A stained by ARID1A antibody [GT3611] (GTX632013) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations