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ARID1A antibody [HL2304]

Recombinant antibody
Anti-ARID1A antibody [HL2304] used in IHC (Paraffin sections) (IHC-P). GTX638371
Anti-ARID1A antibody [HL2304] used in IHC (Paraffin sections) (IHC-P). GTX638371
Anti-ARID1A antibody [HL2304] used in IHC (Paraffin sections) (IHC-P). GTX638371
Anti-ARID1A antibody [HL2304] used in Western Blot (WB). GTX638371

Cat. No. GTX638371

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2304

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ARID1A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

242 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human ARID1A.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AT-rich interaction domain 1A , B120 , BAF250 , BAF250a , BM029 , C1orf4 , CSS2 , ELD , MRD14 , OSA1 , P270 , SMARCF1 , hELD , hOSA1

Cellular Localization

Nucleus

Background

This gene encodes a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ARID1A antibody [HL2304] used in IHC (Paraffin sections) (IHC-P). GTX638371

GTX638371 IHC-P Image

ARID1A antibody [HL2304] detects ARID1A protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
ARID1A stained by ARID1A antibody [HL2304] (GTX638371) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ARID1A antibody [HL2304] used in IHC (Paraffin sections) (IHC-P). GTX638371

GTX638371 IHC-P Image

ARID1A antibody [HL2304] detects ARID1A protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatocellular carcinoma.
ARID1A stained by ARID1A antibody [HL2304] (GTX638371) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ARID1A antibody [HL2304] used in IHC (Paraffin sections) (IHC-P). GTX638371

GTX638371 IHC-P Image

ARID1A antibody [HL2304] detects ARID1A protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
ARID1A stained by ARID1A antibody [HL2304] (GTX638371) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ARID1A antibody [HL2304] used in Western Blot (WB). GTX638371

GTX638371 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ARID1A antibody [HL2304] (GTX638371) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations