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ARID1A antibody

Anti-ARID1A antibody used in Western Blot (WB). GTX129433
Anti-ARID1A antibody used in IHC (Paraffin sections) (IHC-P). GTX129433
Anti-ARID1A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129433
Anti-ARID1A antibody used in IHC (Paraffin sections) (IHC-P). GTX129433
Anti-ARID1A antibody used in Western Blot (WB). GTX129433
Anti-ARID1A antibody used in Immunoprecipitation (IP). GTX129433
Anti-ARID1A antibody used in Western Blot (WB). GTX129433
Anti-ARID1A antibody used in Western Blot (WB). GTX129433

Cat No. GTX129433

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

242 kDa. ( Note )

Positive Control

293T , A431 , HeLa , A549 , HepG2 , HepG2 nuclear extract

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.2M Tris, 0.2M Glycine, 1mM EGTA, 50% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ARID1A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

AT-rich interaction domain 1A , B120 , BAF250 , BAF250a , BM029 , C1orf4 , CSS2 , ELD , MRD14 , OSA1 , P270 , SMARCF1 , hELD , hOSA1

Cellular Localization

Nucleus

Background

This gene encodes a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ARID1A antibody used in Western Blot (WB). GTX129433

GTX129433 WB Image

ARID1A antibody detects ARID1A protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ARID1A antibody (GTX129433) diluted by 1:1000.

Anti-ARID1A antibody used in IHC (Paraffin sections) (IHC-P). GTX129433

GTX129433 IHC-P Image

ARID1A antibody detects ARID1A protein at nucleus on human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
ARID1A antibody (GTX129433) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ARID1A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129433

GTX129433 ICC/IF Image

ARID1A antibody detects ARID1A protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ARID1A protein stained by ARID1A antibody (GTX129433) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-ARID1A antibody used in IHC (Paraffin sections) (IHC-P). GTX129433

GTX129433 IHC-P Image

ARID1A antibody detects ARID1A protein at nucleus on human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
ARID1A antibody (GTX129433) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ARID1A antibody used in Western Blot (WB). GTX129433

GTX129433 WB Image

ARID1A antibody detects ARID1A protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ARID1A antibody (GTX129433) diluted by 1:1000.

Anti-ARID1A antibody used in Immunoprecipitation (IP). GTX129433

GTX129433 IP Image

ARID1A antibody immunoprecipitates ARID1A protein in IP experiments.
IP samples: HepG2 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of ARID1A protein by 4 μg ARID1A antibody (GTX129433)
5 % SDS-PAGE
The immunoprecipitated ARID1A protein was detected by ARID1A antibody (GTX129433) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Anti-ARID1A antibody used in Western Blot (WB). GTX129433

GTX129433 WB Image

ARID1A antibody detects ARID1A protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID1A antibody (GTX129433) diluted by 1:1000.

Anti-ARID1A antibody used in Western Blot (WB). GTX129433

GTX129433 WB Image

ARID1A antibody detects ARID1A protein by Western blot analysis.
A. 30 μg HepG2 whole cell extract
B. 30 μg HepG2 nuclear extract
5 % SDS-PAGE
ARID1A antibody (GTX129433) dilution: 1:1000

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169