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ARID1A antibody

Orthogonal
Anti-ARID1A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129433
Anti-ARID1A antibody used in IHC (Paraffin sections) (IHC-P). GTX129433
Anti-ARID1A antibody used in IHC (Paraffin sections) (IHC-P). GTX129433
Anti-ARID1A antibody used in Western Blot (WB). GTX129433
Anti-ARID1A antibody used in Western Blot (WB). GTX129433
Anti-ARID1A antibody used in IHC (Paraffin sections) (IHC-P). GTX129433
Anti-ARID1A antibody used in Immunoprecipitation (IP). GTX129433
Anti-ARID1A antibody used in Western Blot (WB). GTX129433
Anti-ARID1A antibody used in ChIP assay (ChIP assay). GTX129433

Cat. No. GTX129433

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ARID1A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

242 kDa. ( Note )

Positive Control

A549 , HepG2 nuclear extract

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.53 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ARID1A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885993

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AT-rich interaction domain 1A , B120 , BAF250 , BAF250a , BM029 , C1orf4 , CSS2 , ELD , MRD14 , OSA1 , P270 , SMARCF1 , hELD , hOSA1

Cellular Localization

Nucleus

Background

This gene encodes a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ARID1A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129433

GTX129433 ICC/IF Image

ARID1A antibody detects ARID1A protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ARID1A stained by ARID1A antibody (GTX129433) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Scale bar= 10μm.

Anti-ARID1A antibody used in IHC (Paraffin sections) (IHC-P). GTX129433

GTX129433 IHC-P Image

ARID1A antibody detects ARID1A protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
ARID1A stained by ARID1A antibody (GTX129433) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ARID1A antibody used in IHC (Paraffin sections) (IHC-P). GTX129433

GTX129433 IHC-P Image

ARID1A antibody detects ARID1A protein at nucleus on human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
ARID1A antibody (GTX129433) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ARID1A antibody used in Western Blot (WB). GTX129433

GTX129433 WB Image

ARID1A antibody detects ARID1A protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ARID1A antibody (GTX129433) diluted by 1:1000.

Anti-ARID1A antibody used in Western Blot (WB). GTX129433

GTX129433 WB Image

Orthogonal

ARID1A antibody detects ARID1A protein by Western blot analysis.
A. 30 μg HepG2 whole cell extract
B. 30 μg HepG2 nuclear extract
5 % SDS-PAGE
ARID1A antibody (GTX129433) dilution: 1:1000

Anti-ARID1A antibody used in IHC (Paraffin sections) (IHC-P). GTX129433

GTX129433 IHC-P Image

ARID1A antibody detects ARID1A protein at nucleus on human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
ARID1A antibody (GTX129433) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ARID1A antibody used in Immunoprecipitation (IP). GTX129433

GTX129433 IP Image

ARID1A antibody immunoprecipitates ARID1A protein in IP experiments.
IP samples: HepG2 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of ARID1A protein by 4 μg ARID1A antibody (GTX129433)
5 % SDS-PAGE
The immunoprecipitated ARID1A protein was detected by ARID1A antibody (GTX129433) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-ARID1A antibody used in Western Blot (WB). GTX129433

GTX129433 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID1A antibody (GTX129433) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ARID1A antibody used in ChIP assay (ChIP assay). GTX129433

GTX129433 ChIP assay Image

The data was published in the journal Leukemia in 2019. PMID: 30858552

REFERENCE

Application Reference

Zhiqiang Fu et al. Mol Cell Proteomics 2021; 20 : 100075 Mapping Isoform Abundance and Interactome of the Endogenous TMPRSS2-ERG Fusion Protein by Orthogonal Immunoprecipitation-Mass Spectrometry Assays.
Application : IP
Reactivity : Human
Luca Pagliaroli et al. Nat Commun 2021; 12 (1) : 6469 Inability to switch from ARID1A-BAF to ARID1B-BAF impairs exit from pluripotency and commitment towards neural crest formation in ARID1B-related neurodevelopmental disorders.
Application : WB
Reactivity : Human
Kiminori Kurashima et al. NAR Cancer 2020; 2 (2) : zcaa005 SMARCA4 deficiency-associated heterochromatin induces intrinsic DNA replication stress and susceptibility to ATR inhibition in lung adenocarcinoma.
Application : WB
Reactivity : Human
Sakuratani T et al. Virchows Arch 2020; (Epub) Downregulation of ARID1A in gastric cancer cells: a putative protective molecular mechanism against the Harakiri-mediated apoptosis pathway.
Application : WB
Reactivity : Human
Han L et al. Leukemia 2019; Chromatin remodeling mediated by ARID1A is indispensable for normal hematopoiesis in mice.
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SDS
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169