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ARID2 antibody [GT7311]

KO/KD
Anti-ARID2 antibody [GT7311] used in Western Blot (WB). GTX632011
Anti-ARID2 antibody [GT7311] used in Western Blot (WB). GTX632011
Anti-ARID2 antibody [GT7311] used in Western Blot (WB). GTX632011
Anti-ARID2 antibody [GT7311] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632011
Anti-ARID2 antibody [GT7311] used in Western Blot (WB). GTX632011

Cat. No. GTX632011

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT7311

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ARID2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

197 kDa. ( Note )

Positive Control

MEF

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ARID2. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888275

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AT-rich interaction domain 2 , BAF200 , CSS6 , p200

Cellular Localization

Nucleus

Background

ARID2 is a subunit of the PBAF chromatin-remodeling complex (see BAF180; MIM 606083), which facilitates ligand-dependent transcriptional activation by nuclear receptors (Yan et al., 2005 [PubMed 15985610]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-ARID2 antibody [GT7311] used in Western Blot (WB). GTX632011

GTX632011 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID2 antibody [GT7311] (GTX632011) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ARID2 antibody [GT7311] used in Western Blot (WB). GTX632011

GTX632011 WB Image

KO/KD

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID2 antibody [GT7311] (GTX632011) diluted at 1:1000. (WT: wild type; KO: knockout)

Anti-ARID2 antibody [GT7311] used in Western Blot (WB). GTX632011

GTX632011 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ARID2 antibody [GT7311] (GTX632011) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ARID2 antibody [GT7311] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632011

GTX632011 ICC/IF Image

ARID2 antibody [GT7311] detects ARID2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ARID2 stained by ARID2 antibody [GT7311] (GTX632011) diluted at 1:500.
Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody (GTX112141) diluted at 1:500.
Scale bar= 10μm.

Anti-ARID2 antibody [GT7311] used in Western Blot (WB). GTX632011

GTX632011 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID2 antibody [GT7311] (GTX632011) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169