APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , human ARID2-transfected 293T
Predict Reactivity
Mouse(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human ARID2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885997
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
AT-rich interaction domain 2 , BAF200 , CSS6 , p200
Cellular Localization
Nucleus
Background
ARID2 is a subunit of the PBAF chromatin-remodeling complex (see BAF180; MIM 606083), which facilitates ligand-dependent transcriptional activation by nuclear receptors (Yan et al., 2005 [PubMed 15985610]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
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GTX129443 WB Image
ARID2 antibody detects ARID2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID2 antibody (GTX129443) diluted at 1:3000.
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GTX129443 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID2 antibody (GTX129443) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX129443 ICC/IF Image
ARID2 antibody detects ARID2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ARID2 protein stained by ARID2 antibody (GTX129443) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.
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GTX129443 WB Image
ARID2 antibody detects ARID2 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human ARID2-transfected 293T cells
5 % SDS-PAGE
ARID2 antibody (GTX129443) dilution: 1:5000
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GTX129443 IP Image
ARID2 antibody immunoprecipitates ARID2 protein in IP experiments.
IP samples: HeLa whole cell extract
A. 35 μg HeLa whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of ARID2 protein by 4 μg ARID2 antibody (GTX129443)
D. Immunoprecipitation of ARID2 protein by 4 μg ARID2 antibody (GTX129444)
5 % SDS-PAGE
The immunoprecipitated ARID2 protein was detected by ARID2 antibody (GTX129443) diluted at 1:3000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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GTX129443 IP Image
Immunoprecipitation of ARID2 protein from HepG2 whole cell extracts using 5 μg of ARID2 antibody (GTX129443).
Western blot analysis was performed using ARID2 antibody (GTX129443) diluted at 13000.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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REFERENCE
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REVIEW
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