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ARID2 antibody

KO/KD Overexpression
Anti-ARID2 antibody used in Western Blot (WB). GTX129443
Anti-ARID2 antibody used in Western Blot (WB). GTX129443
Anti-ARID2 antibody used in Immunoprecipitation (IP). GTX129443
Anti-ARID2 antibody used in Immunoprecipitation (IP). GTX129443
Anti-ARID2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129443
Anti-ARID2 antibody used in Western Blot (WB). GTX129443

Cat. No. GTX129443

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ARID2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

197 kDa. ( Note )

Positive Control

human ARID2-transfected 293T cells , 293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ARID2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885997

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AT-rich interaction domain 2 , BAF200 , CSS6 , p200

Cellular Localization

Nucleus

Background

ARID2 is a subunit of the PBAF chromatin-remodeling complex (see BAF180; MIM 606083), which facilitates ligand-dependent transcriptional activation by nuclear receptors (Yan et al., 2005 [PubMed 15985610]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-ARID2 antibody used in Western Blot (WB). GTX129443

GTX129443 WB Image

ARID2 antibody detects ARID2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID2 antibody (GTX129443) diluted at 1:3000.

Anti-ARID2 antibody used in Western Blot (WB). GTX129443

GTX129443 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID2 antibody (GTX129443) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ARID2 antibody used in Immunoprecipitation (IP). GTX129443

GTX129443 IP Image

Immunoprecipitation of ARID2 protein from HepG2 whole cell extracts using 5 μg of ARID2 antibody (GTX129443).
Western blot analysis was performed using ARID2 antibody (GTX129443) diluted at 13000.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-ARID2 antibody used in Immunoprecipitation (IP). GTX129443

GTX129443 IP Image

ARID2 antibody immunoprecipitates ARID2 protein in IP experiments.
IP samples: HeLa whole cell extract
A. 35 μg HeLa whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of ARID2 protein by 4 μg ARID2 antibody (GTX129443)
D. Immunoprecipitation of ARID2 protein by 4 μg ARID2 antibody (GTX129444)
5 % SDS-PAGE
The immunoprecipitated ARID2 protein was detected by ARID2 antibody (GTX129443) diluted at 1:3000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-ARID2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129443

GTX129443 ICC/IF Image

ARID2 antibody detects ARID2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ARID2 protein stained by ARID2 antibody (GTX129443) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Anti-ARID2 antibody used in Western Blot (WB). GTX129443

GTX129443 WB Image

Overexpression

ARID2 antibody detects ARID2 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human ARID2-transfected 293T cells
5 % SDS-PAGE
ARID2 antibody (GTX129443) dilution: 1:5000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169