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ARID2 antibody

KO/KD Overexpression
Anti-ARID2 antibody used in Western Blot (WB). GTX129443
Anti-ARID2 antibody used in Western Blot (WB). GTX129443
Anti-ARID2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129443
Anti-ARID2 antibody used in Western Blot (WB). GTX129443
Anti-ARID2 antibody used in Immunoprecipitation (IP). GTX129443
Anti-ARID2 antibody used in Immunoprecipitation (IP). GTX129443

Cat No. GTX129443

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μl ($329),
25 μl ($169)
See all ARID2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

197 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , human ARID2-transfected 293T

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ARID2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

AT-rich interaction domain 2 , BAF200 , CSS6 , p200

Cellular Localization

Nucleus

Background

ARID2 is a subunit of the PBAF chromatin-remodeling complex (see BAF180; MIM 606083), which facilitates ligand-dependent transcriptional activation by nuclear receptors (Yan et al., 2005 [PubMed 15985610]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-ARID2 antibody used in Western Blot (WB). GTX129443

GTX129443 WB Image

ARID2 antibody detects ARID2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID2 antibody (GTX129443) diluted at 1:3000.

Anti-ARID2 antibody used in Western Blot (WB). GTX129443

GTX129443 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID2 antibody (GTX129443) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ARID2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129443

GTX129443 ICC/IF Image

ARID2 antibody detects ARID2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ARID2 protein stained by ARID2 antibody (GTX129443) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Anti-ARID2 antibody used in Western Blot (WB). GTX129443

GTX129443 WB Image

Overexpression

ARID2 antibody detects ARID2 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human ARID2-transfected 293T cells
5 % SDS-PAGE
ARID2 antibody (GTX129443) dilution: 1:5000

Anti-ARID2 antibody used in Immunoprecipitation (IP). GTX129443

GTX129443 IP Image

ARID2 antibody immunoprecipitates ARID2 protein in IP experiments.
IP samples: HeLa whole cell extract
A. 35 μg HeLa whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of ARID2 protein by 4 μg ARID2 antibody (GTX129443)
D. Immunoprecipitation of ARID2 protein by 4 μg ARID2 antibody (GTX129444)
5 % SDS-PAGE
The immunoprecipitated ARID2 protein was detected by ARID2 antibody (GTX129443) diluted at 1:3000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-ARID2 antibody used in Immunoprecipitation (IP). GTX129443

GTX129443 IP Image

Immunoprecipitation of ARID2 protein from HepG2 whole cell extracts using 5 μg of ARID2 antibody (GTX129443).
Western blot analysis was performed using ARID2 antibody (GTX129443) diluted at 13000.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169