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ARID2 antibody

Anti-ARID2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129444
Anti-ARID2 antibody used in IHC (Paraffin sections) (IHC-P). GTX129444
Anti-ARID2 antibody used in Western Blot (WB). GTX129444
Anti-ARID2 antibody used in Immunoprecipitation (IP). GTX129444
Anti-ARID2 antibody used in Western Blot (WB). GTX129444
Anti-ARID2 antibody used in Western Blot (WB). GTX129444
Anti-ARID2 antibody used in Western Blot (WB). GTX129444

Cat No. GTX129444

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

197 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , HeLa nuclear , human ARID2-transfected 293T

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ARID2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

AT-rich interaction domain 2 , BAF200 , CSS6 , p200

Cellular Localization

Nucleus

Background

ARID2 is a subunit of the PBAF chromatin-remodeling complex (see BAF180; MIM 606083), which facilitates ligand-dependent transcriptional activation by nuclear receptors (Yan et al., 2005 [PubMed 15985610]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-ARID2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129444

GTX129444 ICC/IF Image

ARID2 antibody detects ARID2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ARID2 protein stained by ARID2 antibody (GTX129444) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-ARID2 antibody used in IHC (Paraffin sections) (IHC-P). GTX129444

GTX129444 IHC-P Image

ARID2 antibody detects ARID2 protein on human hepatoma and human normal liver by immunohistochemical analysis.
Sample: (a) Paraffin-embedded human hepatoma (b) Paraffin-embedded human normal liver.
ARID2 antibody (GTX129444) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ARID2 antibody used in Western Blot (WB). GTX129444

GTX129444 WB Image

ARID2 antibody detects ARID2 protein by western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg HeLa nuclear lysate/extract
5% SDS-PAGE
ARID2 antibody (GTX129444) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ARID2 antibody used in Immunoprecipitation (IP). GTX129444

GTX129444 IP Image

ARID2 antibody immunoprecipitates ARID2 protein in IP experiments.
IP samples: HepG2 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of ARID2 protein by 4 μg ARID2 antibody (GTX129444)
5 % SDS-PAGE
The immunoprecipitated ARID2 protein was detected by ARID2 antibody (GTX129444) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Anti-ARID2 antibody used in Western Blot (WB). GTX129444

GTX129444 WB Image

ARID2 antibody detects ARID2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID2 antibody (GTX129444) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ARID2 antibody used in Western Blot (WB). GTX129444

GTX129444 WB Image

ARID2 antibody detects ARID2 protein by western blot analysis.
A. 30 μg 293T
B. 30 μg of human ARID2-transfected 293T cells
5% SDS-PAGE
ARID2 antibody (GTX129444) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ARID2 antibody used in Western Blot (WB). GTX129444

GTX129444 WB Image

ARID2 antibody detects ARID2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ARID2 antibody (GTX129444) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169