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ARID5A antibody [GT1683]

Orthogonal Overexpression
Anti-ARID5A antibody [GT1683] used in IHC (Frozen sections) (IHC-Fr). GTX631939
Anti-ARID5A antibody [GT1683] used in Flow cytometry (FACS). GTX631939
Anti-ARID5A antibody [GT1683] used in Western Blot (WB). GTX631939
Anti-ARID5A antibody [GT1683] used in IHC (Paraffin sections) (IHC-P). GTX631939
Anti-ARID5A antibody [GT1683] used in IHC (Paraffin sections) (IHC-P). GTX631939
Anti-ARID5A antibody [GT1683] used in Western Blot (WB). GTX631939

Cat. No. GTX631939

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1683

Isotype

 IgG2a

Application

 WB, IHC-P, IHC-Fr, FACS

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ARID5A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
FACS 1:50-1:200
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

human ARID5A-transfected 293T , THP-1 nuclear extract

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.14 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ARID5A. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888264

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AT-rich interaction domain 5A , MRF-1 , MRF1 , RP11-363D14

Cellular Localization

Nucleus

Background

Members of the ARID protein family, including ARID5A, have diverse functions but all appear to play important roles in development, tissue-specific gene expression, and regulation of cell growth (Patsialou et al., 2005 [PubMed 15640446]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-ARID5A antibody [GT1683] used in IHC (Frozen sections) (IHC-Fr). GTX631939

GTX631939 IHC-Fr Image

ARID5A antibody [GT1683] detects ARID5A protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse mouse cerebellum.
Green: ARID5A stained by ARID5A antibody [GT1683] (GTX631939) diluted at 1:250.
Red: Calbindin, stained by Calbindin antibody (GTX130856) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-ARID5A antibody [GT1683] used in Flow cytometry (FACS). GTX631939

GTX631939 FACS Image

ARID5A antibody [GT1683] (GTX631939) detects ARID5A protein by flow cytometry analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: ARID5A antibody [GT1683] (GTX631939) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-ARID5A antibody [GT1683] used in Western Blot (WB). GTX631939

GTX631939 WB Image

Orthogonal

THP-1 whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARID5A antibody [GT1683] (GTX631939) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.

Anti-ARID5A antibody [GT1683] used in IHC (Paraffin sections) (IHC-P). GTX631939

GTX631939 IHC-P Image

ARID5A antibody [GT1683] detects ARID5A protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder.
ARID5A stained by ARID5A antibody [GT1683] (GTX631939) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ARID5A antibody [GT1683] used in IHC (Paraffin sections) (IHC-P). GTX631939

GTX631939 IHC-P Image

ARID5A antibody [GT1683] detects ARID5A protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
ARID5A stained by ARID5A antibody [GT1683] (GTX631939) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ARID5A antibody [GT1683] used in Western Blot (WB). GTX631939

GTX631939 WB Image

Overexpression

ARID5A antibody detects ARID5A protein by western blot analysis. Non-transfected (-) and ARID5A -transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARID5A antibody (GTX631939) diluted by 1:10000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations