APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:10000 |
1:20-1:200 |
Not tested in other applications.
Calculated MW
Positive Control
3xFlag-human ARID5A-transfected 293T cells , THP-1 nuclear extract
Predict Reactivity
Mouse, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human ARID5A. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888263
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
AT-rich interaction domain 5A , MRF-1 , MRF1 , RP11-363D14
Cellular Localization
Nucleus
Background
Members of the ARID protein family, including ARID5A, have diverse functions but all appear to play important roles in development, tissue-specific gene expression, and regulation of cell growth (Patsialou et al., 2005 [PubMed 15640446]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
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GTX631938 WB Image
THP-1 whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARID5A antibody [GT921] (GTX631938) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.
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GTX631938 FACS Image
ARID5A antibody [GT921] (GTX631938) detects ARID5A protein by flow cytometry analysis. Sample: THP-1 cell. Black: Unlabelled sample was used as a control. Red: ARID5A antibody [GT921] (GTX631938) dilution: 1:20. Acquisition of 20,000 events were collected for FACS analysis.
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GTX631938 WB Image
ARID5A antibody detects ARID5A protein by western blot analysis. Non-transfected (-) and ARID5A-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARID5A antibody (GTX631938) diluted by 1:5000.
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REFERENCE
There are currently no references for ARID5A antibody [GT921] (GTX631938). Be the first to share your publications with this product.
REVIEW
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