GeneTex
United States (US)

ARL1 antibody

GTX108258_39750_IHC_19011507_613.jpg
GTX108258_39750_WB_19011507_301.jpg
GTX108258_39750_IFA_19011507_866.jpg

Cat No. GTX108258

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

HUVEC

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ARL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Adp Ribosylation Factor Like Gtpase 1,Arfl1,Arl1

Cellular Localization

Golgi apparatus membrane

Background

The protein encoded by this gene belongs to the ARL (ADP-ribosylation factor-like) family of proteins, which are structurally related to ADP-ribosylation factors (ARFs). ARFs, described as activators of cholera toxin (CT) ADP-ribosyltransferase activity, regulate intracellular vesicular membrane trafficking, and stimulate a phospholipase D (PLD) isoform. Although, ARL proteins were initially thought not to activate CT or PLD, later work showed that they are weak stimulators of PLD and CT in a phospholipid dependent manner. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX108258_39750_IHC_19011507_613.jpg

GTX108258 IHC-P Image

ARL1 antibody detects ARL1 protein at cytoplasm on human liver carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded liver carcinoma.
ARL1 antibody (GTX108258) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108258_39750_WB_19011507_301.jpg

GTX108258 WB Image

Sample (30 ug of whole cell lysate)
A: HUVEC
12% SDS PAGE
GTX108258 diluted at 1:1000

GTX108258_39750_IFA_19011507_866.jpg

GTX108258 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using ARL1(GTX108258) antibody at 1:500 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169